PHLEBOTOMY OF NORMAL VOLUNTEERS TO SUPPORT PLASMODIUM FALCIPARUM

每周从健康志愿者身上收集的红细胞已经被使用 维持疟疾寄生虫(疟原虫)的体外培养 恶性疟原虫)。这些生物已经在一系列实验室中使用 学习。一方面，需要对抗疟疾药物进行体外评估 青蒿素合成类似物的效力。从这些研究中，我们得到了 对这一作用的分子机制有了新的见解 重要的一类特工。培养物也被用来分离DNA 和来自恶性疟原虫的RNA，以监测热休克的产生 蛋白质，用来感染蚊子，并在旨在评估 候选疫苗。最后，这些培养物被用来感染 一项研究中使用的蚊子，以确定阿托瓦酮 可以预防人类的疟疾。

Red blood cells collected weekly from healthy volunteers have been used to sustain in vitro cultures of malaria parasites (Plasmodium falciparum). These organisms have been used in an array of laboratory studies. One aspect entails the in vitro evaluation of antimalarial potency of synthetic analogs of artemisinin. From these studies we have gained new insights into the molecular mechanism of action of this important class of agents. Cultures have also been used to isolate DNA and RNA from P. falciparum, to monitor the production of heat shock proteins, to infect mosquitoes, and in studies designed to evaluate vaccine candidates. Finally, these cultures were used to infect mosquitoes that were used in a study to determine whether atovaquone could prevent malaria in humans.