DEVELOPMENT OF A NEW METHOD FOR ISOLATING THE C-TERMINAL PROTEOLYTIC PEPTIDE

一种刻画C-三元系的新方法 蛋白质已经被开发出来了。该战略包括三个步骤。 首先，用胰酶或Lys-C对蛋白质进行酶降解。 其次，将C-藤脑肽与所有其他多肽分离 蛋白水解物通过使用无氢胰蛋白酶，一种改良的 仅与C末端含有K或R的多肽结合的胰酶。 最后，用MALDI对C端肽进行了表征。这个 该策略在一系列设计的 合成肽以及转录因子毛发，其中 C-末端多肽易于分离和鉴定。

A new approach for the characterization of the C-terniiiials of proteins has been developed. The strategy involves three steps. First, the protein is degraded enzymatically with trypsin or Lys-C. Second the C-tenninal peptide is separated from all the other proteolysis products through the use of anhydrotrypsin, a modified trypsin that binds only peptides containing K or R at the C-terminus. Finally, the C-terminal peptide is characterized by MALDI. The strategy was tested and shown to work well on a series of designed synthetic peptides as well as on the transcription factor Hairy, where the C-terminal peptide was readily isolated and identified.