XRAY FLUORESCENCE STUDIES OF NI CONTAINING CARBON MONOXIDE DEHYDROGENASE ENZYMES

一氧化碳脱氢酶是一种在氧化还原过程中的酶。 碳固定和当地电子和物理知识 这些酶中金属簇的结构对于 对一氧化碳氧化和乙酰辅酶A机理的认识 综合。高能钾荧光的探测 分辨率揭示了有关氧化和自旋态的信息 第一排过渡金属化合物。我们建议使用 用K_荧光的自旋态灵敏度研究CODH 从红色红曲霉中提取酶以扩展目前对镍-铁的认识 通过量化高自旋和低自旋镍的数量来实现金属团簇 现在时。我们计划使用位置选择性x射线吸收来研究一种 Ni-Fe中心(A中心，乙酰辅酶A合成中心)在 高温醋酸菌研究镍(I)和镍(I)的配位环境 Ni(II)，并确定CO是否与Ni结合。

Carbon monoxide dehydrogenases are enzymes in the process of carbon fixation and knowledge of the local electronic and physical structure of the metal clusters in these enzymes is essential for the understanding of the mechanisms of CO oxidation and acetyl-CoA synthesis. The detection of the K _ fluorescence with high energy resolution reveals information about oxidation and spin states of first-row transition metal compounds. We propose to use the spin-state sensitivity of the K _ fluorescence to investigate the CODH enzyme from R. rubrum to extend the current understanding of the Ni-Fe metal cluster by quantifying the amount of high and low spin Ni present. We plan to use site-selective x-ray absorption to study one of the Ni-Fe centers (Center A, the site of acetyl-CoA synthesis) in C. thermo-aceticum CODH to study the ligand environment of Ni(I) and Ni(II) and to determine if CO binds to Ni.