STRUCTURE DETERMINATION OF CATALYTIC, METAL BINDING & ANTI BACTERIAL ANTIBODIES

我们使用的是43c9抗体，它是针对一种 磷酰胺半抗原催化酰胺和酯的水解性 作为脚手架的邦德，为设计金属场地提供了终极 目标是设计一个具有催化活性的金属站点。 结晶学中侧链位置的差异 天然43c9和一个锌结合突变体的结构已被用于 确定锌结合部位的残基。此外，我们还有 能够基于结晶学对半抗原结合进行建模 结构43c9-对-硝基苯酚二元络合物，并用其鉴定 其他将改善金属结合部位的突变。在 未来，我们希望进一步探索这个锌结合部位，并 阐明其他潜在的催化金属结合的结构 网站。

We are using the 43C9 antibody, which was raised against a phosphonamidate hapten to catalyze the hydrolysis of amide and ester bonds, as scaffold for the design of metal sites with the ultimate goal of designing a site with a catalytically active metal. Differences in side chain positions between the crystallographic structures of native 43C9 and a Zn-binding mutant have been used to identify residues that will the Zn-binding site. In addition, we have been able to model the hapten binding based on the crystallographic structure 43C9-p-nitrophenol binary complex, and used it to identify other mutations that will improve the metal binding site. In the future we hope to explore this Zn-binding site further and to elucidate the structures of other potential catalytic metal binding sites.