TERNARY STRUCTURE OF CYTOCHROME P450BM 3 HEME DOMAIN CO COMPLEX

含底物的细胞色素P450BM-3结构域2.7E数据集 Bound于1996年5月在SSRL收集，并解决了结构问题 通过分子置换。这个结构揭示了一个很大的 底物结合时蛋白质骨架的构象变化 这导致衬底访问通道关闭。我们是 进一步筛选出更好的防冻剂和降凝剂 条件下，也将尝试通过与 戊二醛。三元晶体结构的测定 P450BM-3血红素结构域-底物-CO复合体可能是唯一途径 将血红素锁定在可能的底物所在的亚铁状态 可以检测到构象变化。

A 2.7 E data set of cytochrome P450BM-3 heme domain with substrate bound was collected in May, 1996 at SSRL and the structure was solved by molecular replacement. This structure reveals a large conformational change in protein backbone upon the substrate binding which results in the closure of the substrate access channel. We are going to further screen better cryo-protectant and reducing conditions, also will try to stabilize crystals by cross linking with glutaraldehyde. Determination of a ternary crystal structure of P450BM-3 heme domain-substrate-CO complex might be the only way to lock the heme in its ferrous state from which the possible substrate conformational changes can be detected.