HI RES DATA OF NATIVE DIHEME CYTOCHROME C PEROXIDASE FROM NITROSOMONAS EUROPAEA

欧洲亚硝化单胞菌天然二血红素细胞色素 C 过氧化物酶的高分辨率数据

• 批准号: 6586534
• 负责人: HUIYING LI
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586534
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

The diheme cytochrome c peroxidase from Nitrosomonas europeae has been successfully crystallized in our lab and data have been collected to a resolution of 2.9 E. The normal data collection on our Siemens area detector takes four to five days, preventing us from getting good anomalous signals that could be used to locate the heme irons. We are hoping that not only will we extend our data to a higher resolution but also the rapid data collection with synchrotron radiation and MAR-Research imaging plate will provide us with high quality anomalous dispersion data which will aid the phase calculation.

来自欧洲亚硝化单胞菌的二血红素细胞色素c过氧化物酶具有 在我们的实验室中成功结晶，并收集了数据 分辨率为2.9E。西门子的常规数据收集 区域探测器需要四到五天的时间， 可以用来定位血红素的异常信号 我们 希望我们不仅能将数据扩展到更高的分辨率， 还包括同步辐射的快速数据收集， MAR-Research成像版材将为我们提供高质量的异常 色散数据，这将有助于相位计算。