DATA COLL ON CO CRYSTAL BETWEEN ACTIVE RAS & RAS INTERACTING DOMAIN OF RALGDS

Ras蛋白是一种鸟苷三磷酸酶（GTdR），是一种分子 在控制细胞生长的信号转导途径中的转换， 分化 在人类细胞中，关键区域的点突变 可以导致不受控制的细胞生长或肿瘤。 我们正在努力 解决活性Ras蛋白之间复合物的结构 （Ras-GMPPNP）和RalGDS的Ras相互作用结构域（RalGDS-RID），a Ral蛋白（Ras样蛋白）的GDP解离刺激剂。 我们 实验室已经解决了活性Ras结构和 RalGDS-RID与MAD方法。 我们想得到一种共晶 这将使我们能够解决复杂的结构， 分子置换法

Ras protein, a guanosine triphosphatase (GTPase), is a molecular switch in signal transduction pathways that control cell growth and differentiation. In human cells, point mutations in crucial regions of Ras can cause uncontrolled cell growth, or tumors. We are trying to solve the structure of the complex between active Ras protein (Ras-GMPPNP) and the Ras interacting domain of RalGDS (RalGDS-RID), a GDP dissociation stimulator of Ral protein (Ras like protein). Our lab has solved the active Ras structure and the structure of RalGDS-RID with the MAD method. We would like to obtain a co-crystal data set, which will allow us to solve the structure of the complex by the Molecular Replacement method.