STRUCTURE & DYNAMICS OF CENTRIOLE

(部分由CNRS向M.Bornens提供的赠款支持) 人中心体/中心粒复合体结构的研究 通过各种方法进行研究，包括孤立的IVEM断层扫描 中心粒镶嵌在阴性染色中。因为中心粒 非常小(0.25乘0.5米)和复杂，唯一的方法是它们 形态可以用EM来研究。通常，这些细胞器是 在常规塑料嵌入试件的切片上进行检查。 使用这种技术获得的分辨率有一定的局限性。Dr。 鲁伊斯分离了人类中心体，并一直在研究它们 阴性染色。她获得了高分辨率的图像，显示出 中心粒结构的周期性。然而，要研究 3-D结构她需要BMIRR的IVEM自动断层扫描系统 由于整装制剂的厚度和 阴性染色制剂的剂量敏感性。当在 她还介绍了在住处进行断层扫描的用途。 冷冻水合中心粒。在她的访问中，鲁伊斯博士记录了六个 双倾斜系列和一个单倾斜断层成像系列。一个测试 重建是由Rath博士完成的。该项目将继续 雷德马赫博士的协助，并于今年抽象出来：鲁伊斯， T.，M.Radermacher，B.Rath，C.Rieder和M.Bornens(1998) 中心体：40A时细胞器的三维结构 通过电子层析成像获得的分辨率。显微镜和 微量分析4：436-437。

(Supported in part by CNRS grants to M. Bornens) In this ongoing study of the structure of the human centrosome/centriole complex is being studied by various methods including IVEM tomography of isolated centrioles that are embedded in negative stain. Because centrioles are extremely small (0.25 by 0.5 (m) and complex, the only way their morphology can be studied is with EM. Normally, these organelles are examined in sections of conventionally plastic embedded specimens. The resolution obtained using this technique is somewhat limited. Dr. Ruiz has isolated human centrosomes and has been studying them after negative staining. She has obtained high-resolution images showing the periodicity of the centriolar structure. However, to study the 3-D structure she needed the BMIRR's IVEM automated tomography system because of the thickness of the whole-mount preparations and the dose-sensitivity of the negative-stain preparations. While in residence she also explo red the u tility of conducting tomography on frozen-hydrated centrioles. On her visit Dr. Ruiz recorded six double-tilt series and one single-tilt tomographic series. A test reconstruction was made by Dr. Rath. This project will continue with the assistance of Dr. Radermacher and was abstracted this year: Ruiz, T., M. Radermacher, B. Rath, C. Rieder and M. Bornens (1998) The centrosome: three-dimensional structure of a cell organelle at 40A resolution obtained by electron-tomography. Microscopy and Microanalysis 4:436-437.