STRUCTURE & DYNAMICS OF CENTROSOME

（部分由CNRS赠款M。这是一种 一项正在进行的关于人类中心体结构的研究 因为 中心体非常小（0.25 × 0.5（m））和复杂， 研究它们形态的方法是用EM。 通常，中心体 在常规包埋和观察后，在EM中进行检查 塑料型材 可获得的分辨率在某种程度上是有限的， 这种技术。 我们的实验室已经分离出了人类中心体， 在负染色后研究它们。 我们所获得 显示中心粒周期性的高分辨率图像 结构 为了研究三维结构，我们需要自动化的IVEM 由于整体支架的厚度， 制剂和负染剂的剂量敏感性 准备工作 我们也在考虑未来的冷冻水化工作。 在她的访问博士鲁伊斯记录了六个双倾斜系列和一个 单倾斜层析成像系列。 一个测试重建是由博士。 拉特。 该项目将继续与博士的协助。 拉德马赫

(Supported in part by CNRS grants to M. Bornens) This is part of an ongoing study of the structure of the human centrosome. Because centrosomes are extremely small (0.25 by 0.5 (m) and complex, the only way their morphology can be studied is with EM. Normally, centrosomes are examined in the EM after embedding and observation in conventional plastic sections. The resolution obtainable is somewhat limited using this technique. Our lab has isolated human centrosomes, and we have been studying them after negative staining. We have obtained high-resolution images showing the periodicity of the centriolar structure. To study the 3-D structure, we needed the IVEM automated tomography system because of the thickness of the whole-mount preparations and the dose-sensitivity of the negative-stain preparations. We also contemplate frozen-hydrated work in the future. On her visit Dr. Ruiz recorded six double-tilt series and one single-tilt tomographic series. A test reconstruction was made by Dr. Rath. This project will continue with the assistance of Dr. Radermacher.