IMMUNO LABELING OF CALCIUM RELEASE CHANNEL PROTEIN

（由NIH AR 40615向T. Wagenknecht）的主要目标 我们的小组要确定兰尼碱受体的结构 (RyR)进行兴奋-收缩的蛋白质组装 骨骼肌和心肌中的偶联。 我们使用的主要工具是 冷冻电子显微镜和图像处理。 我们已经标记了 配体与纳米金的受体，如果结合是 成功的，玻璃化标本的高分辨率EM图像 应该显示RyR与特定位置的连接探针。 这种配体，一种肽毒素，对开放/封闭的 频道的状态。 我们正在进行的实验保证 提供了对肌肉刺激机制的新见解， 神经引起收缩，这是一个非常感兴趣的领域， 生物医学科学家，因为它对理解 以及治疗包括心脏在内的肌肉疾病。 我们确定了 IVEM上这项工作的最佳成像和记录条件， 这将用于补充CTEM所做的工作。 数据 开始收集，取得了良好的效果。

(Supported by NIH AR40615 to T. Wagenknecht) The main objective of our group is to determine the structure of the ryanodine receptor (RyR), the protein assembly that carries out excitation-contraction coupling in skeletal and heart muscle. The main tools we use are cryo-electron microscopy and image processing. We have labeled a ligand to the receptor with nanogold and, if the binding is successful, the high-resolution EM images of the vitrified specimen should show the RyR with the attached probe at a specific location. This ligand, a peptide toxin, has a direct effect on the open/closed state of the channel. The experiments we are carrying out promise to provide new insights into the mechanism by which stimulation of muscle by nerve leads to contraction, an area that is of intense interest to biomedical scientists because of its implications for understanding and treating muscular, including heart, diseases. We determined the optimal imaging and recording conditions for this work on the IVEM, which will be used to supplement the work done with CTEM. Data collection was started, with good results.