DYNAMIC MACROMOLECULAR STRUCTURES IN SOLUTION VIA ANALYSIS OF NMR EXPERIMENTS

硫酸盐化是一种广泛存在的翻译后修饰 存在于所有含有高尔基体的动物细胞中的酪氨酸。在……里面 事实上，大约1%的酪氨酸在组织中被硫酸盐化 老鼠。尽管如此，只有24个独特的蛋白质序列 已描述了含有酪氨酸硫酸盐的化合物。这样做的目的是 项目是分析KNON周围的氨基酸序列 酪氨酸硫化位点确定该位点的特征 因此，确定更多的硫酸盐化候选者。这个 具体目标是：1.确定硫酸盐的特征 酪氨酸，区别于非硫酸盐化的酪氨酸。这些 特征包括氨基酸的数量和分布，如 以及附近可能的次级构造特征。 酪氨酸。2.鉴定可能含有酪氨酸的蛋白质 来自瑞士蛋白质数据库的硫酸盐化位点。3.确定 附近一组已知蛋白质之间的序列相似性 硫酸盐化地点的。新的蛋白质硫酸盐化位点的鉴定 对于理解这些分子的功能可能是必不可少的 并将有助于更好地理解蛋白质的功能。 酪氨酸硫酸盐化。

Sulfation is a widespread post-translational modification of tyrosines found in all animal cells containing a Golgi system. In fact, approximately 1% of the tyrosines are sulfated in tissue in the rat. Despite this observation, only 24 unique protein sequences containing tyrosine sulfate have been described. The aim of this project is to analyze the amino acid sequences surrounding know tyrosine sulfation sites to determine the characteristics of this site and, therefore, to identify more candidates of sulfation. The specific goals are: 1. to identify the characteristics of sulfated tyrosines which distinguish them from non-sulfated tyrosines. These characteristics include the number and distribution of amino acids, as well as the possible secondary structural features in the vicinity of the tyrosine. 2. to identify proteins with probable tyrosine sulfation sites from the Swiss Protein data base. 3. to determine sequence similarities among the set of known proteins in the vicinity of the sulfation site. Identification of new protein sulfation sites may be essential for the understanding of the function of these proteins and will lead to a better understanding of the function of tyrosine sulfation.