PHYTOREMEDIATION OF TOXIC WASTE SITES

我们计划确定植物修复有毒废物场地的能力。 在原型实验中，我们将重点关注极地树的能力， 消除三氯乙烯（TCE）的毒性。 杨树能够在 高浓度的三氯乙烯并使其蒸发和代谢。 TCE 转化为三氯乙醇，三氯乙酸和两种极性物质。 其结构将被确定。 我们将决定 无性系代谢植物吸收的TCE的最大比例。 新陈代谢的程度将通过引入基因来增强， 已知能代谢三氯乙烯 摄取和代谢的实验室研究 的TCE将使用带有单独腔室的气密玻璃装置， 根和冠，以确定100的材料的命运 管理。 最好的克隆将在温室规模进行研究 实验，以确定他们的能力，吸收和解毒三氯乙烯。 最后，将与 西方化学公司在塔科马港的一个地点。 这些 实地研究将进行2至4年，将大量 仪器，将决定植物的吸收能力， 解毒TCE，并将测量化合物的蒸腾程度。 结合这些研究，我们将与Leah Bauer博士合作， USDA，东兰辛，MI，以确定雏鸡摄入的影响 以确定是否对这些动物有任何影响。 我们还将研究我们是否可以利用植物的能力， 新陈代谢 TCE作为环境生物标志物 地下TCE污染的程度。 植物组织从一系列的 将分析研究中心的TCE代谢物，并与已知的 三氯乙烯污染。 利用植物修复污染符合NIEHS的要求， 减少有毒物质对人类健康的不利影响。 这 作为一种“绿色解决方案”，以减少污染， 希望得到公众的认可。

We plan to determine the ability of plants to remediate toxic waste sites. In prototype experiments, we will focus on the ability of polar trees to detoxify trichloroethylene (TCE). Poplars are able to grow in the presence of high levels of TCE and to transpire and metabolize the compounds. TCE is converted to trichloroethanol, trichloroacetic acid, and two polar compounds whose structures will be determined. We will determine which clones metabolize the largest proportion of TCE absorbed by the plants. The extent of metabolism will be augmented by introducing genes which are known to metabolize TCE. Laboratory studies of the uptake and metabolism of TCE will use gas-tight glass apparatuses with separate chambers for roots and crowns in order to determine the fate of 100: of the material administered. The best clones will be studied in greenhouse-scale experiments to determine their ability to absorb and detoxify TCE. Finally, field studies will be conducted in collaboration with the Occidental Chemical Corporation at a site at the Port of Tacoma. These field studies will take place for 2 to 4 years, will be heavily instrumented, will determine the ability of the plants to take up and detoxify TCE, and will measure the extent of transpiration of the compound. In conjunction with these studies, we will collaborate with Dr. Leah Bauer, USDA, East Lansing, MI, to determine the effect of the ingestion of chicks to determine whether there is any effect on these animals. We will also investigate whether we can use the ability of plants to metabolize. TCE to chlorinated products as an environmental biomarker of the extent of below-ground TCE contamination. Plant tissue from a range of sites will be analyzed for TCE metabolites and compared to known levels of TCE contamination. The use of plants to remediate pollution meets the mandate of the NIEHS to attenuate adverse effects of toxic materials on human health. This approach serves as a "green solution" to pollution abatement and is expected to meet with public approval.