Peptidomics and Proteomics Core Facility fast scanning high resolution mass spectrometer

Wellcome-MRC代谢科学研究所的肽组学和蛋白质组学核心专门鉴定和测量血浆和组织样本中的肽和小蛋白质。血液中肽和蛋白质的分析特别具有挑战性，因为感兴趣的蛋白质可能以非常低的水平存在，并且有许多其他蛋白质以非常高的水平存在，这些蛋白质可以作为掩模。如果不首先去除这些高丰度蛋白质，检测低丰度肽和蛋白质是非常困难的。我们的实验室已经开发出特定的方法来去除这些高丰度的蛋白质，这样我们就可以检测到浓度低得多的较小的肽和蛋白质。胰岛素在血液中的含量比白蛋白低十亿倍左右，但是我们的方法和我们现有的质谱仪相结合，使我们能够在这种低浓度下检测胰岛素，尽管有白蛋白背景。不幸的是，这是我们现有仪器的灵敏度极限（50 pg/mL），然而还有许多其他肽的循环水平远远低于胰岛素，我们很难检测到自从我们购买了现有的质谱仪以来，技术上有了重大的进步，新仪器将使我们能够检测血液中较低浓度的肽和激素。新仪器将用于一系列研究，其中许多集中在确定代谢疾病的原因和潜在的新治疗方法，包括2型糖尿病，肥胖和癌症恶病质，并开发新的肿瘤和肠道疾病诊断测试。购买更新，更快，更强大的仪器的能力将使肽组学和蛋白质组学核心在未来十年大大改善服务。

The Peptidomics and Proteomics Core at the Wellcome-MRC institute of Metabolic Science specialises in identifying and measuring peptides and small proteins in plasma and tissue samples. The analysis of peptides and proteins in blood is particularly challenging because the protein of interest might be present at very low levels, and there are a number of other proteins found at very high levels that act as a mask. Detecting the lower abundant peptides and proteins is very difficult without removing these high abundance proteins first. Our laboratory has developed specific methods to remove these high abundant proteins so that we can detect the smaller peptides and proteins present at much lower concentrations.A good example of this is the hormone insulin. This is present in blood at levels around a billion times lower than albumin, but the combination of our methods and our current mass spectrometer enable us to detect insulin at this low concentration despite the albumin background.Unfortunately this is the limit of the sensitivity of our existing instrument (50pg/mL), yet there are many other peptides that circulate at levels far lower than insulin, which we struggle to detect at all. Since we purchased our current mass spectrometer, there have been significant advances in technology, and the new instruments will enable us to be able to detect peptides and hormones present at lower concentrations in blood.The new instrument will be used for a range of studies, many focussed on identifying the causes and potential new treatments for metabolic diseases including type 2 diabetes, obesity and cancer cachexia, and developing new diagnostic tests for tumours and intestinal diseases. The ability to purchase a newer, faster and more capable instrument will give the Peptidomics and Proteomics Core a vastly improved service for the next decade.