GENETICS AND BIOCHEMISTRY OF RIBOSOME SYNTHESIS

描述（改编自研究者摘要）：核糖体， 蛋白质合成所必需的一种机制， 在细胞生长和它的合成是密切相关的 原核和真核细胞增殖的调控 细胞 当通过各种手段刺激细胞增殖时， 核糖体合成的增加是一种非常早期的 在实际细胞增殖发生之前的事件。 这个目标 一个项目是了解细胞如何调节核糖体的产生 以应对环境条件。 已知的是， 核糖体RNA（rRNA）是决定整体合成的核心。 核糖体，因此，研究人员将专注于调节 RNA的合成。 PI将使用酿酒酵母 作为一个模型系统，因为基因操作的容易性和 RNA聚合酶合成rRNA的可用知识1. 他将 继续鉴定参与 参与的这些组成部分的状态的调节 rRNA合成的调控。 为了阐明 调控，他将分析这些组件的状态，rDNA 模板、RNA聚合酶1和各种转录因子 高和低rRNA合成。 核仁，核糖核酸的位置 合成和核糖体组装，对细胞生长显然很重要， 但其结构复杂，其功能重要性可能不 非常感谢 事实上，最近的研究表明， 细胞老化 除了研究rRNA的合成，他还将 研究酵母细胞核仁的结构和定位。 的 拟议的工作旨在为理解 重要的核仁功能，包括核糖体的合成， 调控

DESCRIPTION (adapted from investigator's abstract): The ribosome, the machinery essential for protein synthesis, obviously plays a key role in cell growth and its synthesis is intimately connected to the regulation of cell proliferation both in prokaryotic and eukaryotic cells. When cells are stimulated to proliferate by various means, an increase in ribosome synthesis takes place as one of the very early events before actual cell proliferation occurs. The goal of this project is to understand how cells regulate the production of ribosomes in response to environmental conditions. It is known that synthesis of ribosomal RNA (rRNA) is central in determining overall synthesis of ribosomes, and hence, the investigator will concentrate on regulation of rRna synthesis. The PI will use the yeast Saccharomyces cerevisiae as a model system because of the ease of genetic manipulation and the available knowledge of rRna synthesis by RNA polymerase 1. He will continue to identify molecular components participating in the regulation of the state of these components participating in the regulation of rRNA synthesis. To elucidate the mechanisms of regulation, he will analyze the state of these components, the rDNA template, RNA polymerase 1, and various transcription factors, in cells with high and low rRNA synthesis. The nucleolus, the site for rRna synthesis and ribosome assembly, is obviously important for cell growth, but its structure is complex and its functional importance may not be fully appreciated. In fact, recent studies suggest its importance in cell aging. In addition to studies of rRNA synthesis, he will also study the structure and localization of the nucleolus in yeast. The proposed work is intended to make a contribution to the understanding of important nucleolar functions including ribosome synthesis and it's regulation.