INGESTIVE BEHAVIOR IN SEROTONIN RECEPTOR MUTANT MICE

描述：(改编自《调查者摘要》)此应用程序是一个 申请独立科学家奖(K02)以促进研究事业 劳伦斯·特科特博士的发展。泰科特博士的长期目标是将 确定个体贡献的分子遗传学方法 复合体5-羟色胺受体亚型对5-羟色胺能调节的影响 行为。这项工作的一个主要重点将是开发遗传和 确定神经机制的行为方法 5-羟色胺5-HT2C和5-HT1A受体亚型调节食物摄入。初步 有证据表明，5-HT2C受体突变小鼠存在摄食失调， 导致肥胖的发展。5-HT1a受体的初步研究 突变的小鼠增加了这些动物大脑过度活跃的可能性 血清素系统。5-HT2C和5-HT1A型受体对血管内皮细胞生长的影响 调节进食的神经心理过程将与 它们对神经递质系统的影响参与调节 喜庆的行为。 独立科学家奖将允许特科特博士开发新的技能和 追求他的研究目标的方法。例如， 研究计划是开发小鼠的摄食行为分析方法。 这些努力将促进转基因技术在 研究喂养并使特科特博士获得对数量的欣赏 评估行为的方法。的另一个重要目标 研究计划是开发产生5-羟色胺受体的程序 仅限于特定大脑区域和神经化学的突变 确定了细胞类型。这种方法将有助于识别神经 5-羟色胺受体影响复杂行为的结构。这些 目标将在优秀合作者的指导下实现，允许 Tecott博士将开发专业知识，以补充他现有的 神经解剖学和分子生物学技能。

DESCRIPTION: (Adapted from the Investigator's Abstract) This application is a request for an Independent Scientist Award (K02) to further the research career development of Dr. Laurence Tecott. Dr. Tecott's long term goal is to apply molecular genetic approaches to determine the contributions of individual serotonin receptor subtypes to the serotonergic regulation of complex behaviors. A major focus of this work will be the development of genetic and behavioral approaches for determining the neural mechanisms through which the serotonin 5-HT2C and 5-HT1A receptor subtypes regulate food intake. Preliminary evidence reveals a dysregulation of feeding in 5-HT2C receptor mutant mice, leading to the development of obesity. Preliminary studies of 5-HT1A receptor mutant mice raise the possibility that these animals have hyperactive brain serotonin systems. The impact of 5-HT2C and 5-HT1A receptors on the neuropsychological processes that regulate feeding will be correlated with their effects on neurotransmitter systems implicated in the regulation of ingestive behavior. An Independent Scientist Award will allow Dr. Tecott to develop new skills and approaches for pursuing his research goals. For example, a major focus of the research plan is the development of ingestive behavior assays for the mouse. These efforts will facilitate the application of transgenic technologies to the study of feeding and enable Dr. Tecott to gain an appreciation of quantitative approaches to the assessment of behavior. Another important goal of the research plan is the development of procedures for generating 5-HT receptor mutations that are restricted to particular brain regions and neurochemically identified cell types. Such approaches will be useful for identifying neural structures through which 5-HT receptors influence complex behaviors. These goals will be pursued with guidance from outstanding collaborators, allowing Dr. Tecott to develop expertise that will complement his existing neuroanatomical and molecular biology skills.