COCHLEAR BLOOD FLOW AND NEUROPEPTIDES

偏头痛相关的内耳症状，如发音障碍、耳鸣、听力波动、听力损失和噪声敏感性增加，为基底动脉偏头痛和耳蜗病理生理机制之间可能的神经学基础提供了证据。最近，我们发现了一个以前从未报道过的感觉神经支配的耳蜗血管起源于三叉神经节。我们已经表明，这种感觉神经支配在正常和病理条件下（例如，在内淋巴积水（梅尼埃病的症状之一）的动物模型中）。该建议旨在进一步确定椎血管和耳蜗血管系统周围三叉神经感觉网络的解剖学基础和机制。该提案提出了这样的假设，即三叉神经感觉系统及其相关的神经肽系统是导致基底偏头痛和耳蜗血管稳态的重要因素。这项研究有三个具体目标。目标1.确定基底动脉偏头痛的耳蜗症状是否有生理学基础。阳性结果将证实基底节偏头痛和耳蜗症状的共同功能基础，该基础可能是神经源性炎症。目标二。目的：探讨香草酸受体（VR 1）和P物质（SP）在耳蜗血管、基底动脉及其相关分支的分布情况。阳性免疫细胞化学结果将证明：（a）VR 1和（B）SP共标记的基底动脉、小脑前下动脉（AICA）、螺旋蜗轴动脉（SMA）和桡动脉周围的初级感觉神经元的网络;（c）辣椒素将导致标记感觉纤维密度的显著降低。目标3。目的探讨内淋巴积水三叉神经感觉神经元的血管调节功能障碍。在这项研究中，积极的结果将证明，内淋巴积水导致减少刺激三叉神经节诱导的CBF变化。该研究将有助于阐明三叉神经感觉神经元在正常和病理条件下如何调节椎-基底动脉血管系统和耳蜗液体平衡。

The migraine related inner ear symptoms for phonopobia , tinnitus, hearing fluctuation, hearing loss, and increased noise sensitivity provide evidence for a possible neurological substrate connecting basilar artery migraine and cochlear pathophysiological mechanisms. Recently we have identified a previously unreported sensory innervation of the cochlear blood vessels originating from the trigeminal ganglia. We have shown that this sensory innervation has a significant effect on cochlear blood flow (CBF) in both normal and pathological conditions (e.g., in the animal model of endolymphatic hydrops, one of the symptoms of Meniere's disease). This proposal seeks to further define the anatomical basis and mechanisms of the trigemino-sensory network around the vertebrovasilar and cochlear vascular system. The proposal offers the hypothesis that the trigemino-sensory system and its related neuropeptide system are important factors contributing to basilar migraine and vascular homeostasis of the cochlea. The study has three specific aims. Aim 1. To establish if there is a physiological basis for the cochlear symptoms in basilar artery migraine headache. Positive results will confirm a common functional basis for basilar migraine and cochlear symptoms, the basis could be neurogenic inflammation. Aim 2. To demonstrate if vanilloid receptor (VR1) and substance P (SP) are co-localized around cochlear blood vessels, the basilar artery and its related branches. Positive immunocytochemical results will demonstrate: (a) network of the VR1 and (b) SP co-labeled primary sensory neurons around the basilar artery; anterior inferior cerebellar artery (AICA), spiral modiolar artery (SMA) and radial artery; (c), Capsaicin will cause a significant reduction in the density of labeled sensory fibers. Aim 3. To determine the vasoregulatory disturbance of the trigemino-sensory neurons in endolymphatic hydrops. In this study positive results will demonstrate that endolymphatic hydrops causes a reduction in the stimulated trigeminal ganglion induced CBF change. The studies of the proposal will help clarify how trigemino-sensory neurons regulate the vertebro-basilar vascular system and cochlear fluid balance under normal and pathological conditions.