DIVERSITY OF CTL EPITOPES AND CHLAMYDIA

衣原体是细菌性传播的最常见原因 发达国家的疾病，导致明显的疾病和 不孕不育，也是可预防失明的主要原因 全世界。衣原体自然免疫及其疫苗策略 到目前为止已经尝试的只提供了有限的保护 血型。这些研究的目标是确定疫苗是否 启动细胞毒性T淋巴细胞(CTL)反应的策略可以提供 对广泛的衣原体血清型的保护。我们已经培养了 来自感染小鼠的衣原体反应性CTL，并已表明它们是 对衣原体感染细胞的特异性和体外裂解作用。在领养时 将这些CTL转移到衣原体感染的小鼠体内，减少了 可以在脾中测量细菌载量。在中国的初步实验 这项提案将决定衣原体特异性CTL是否可以 在不同MHC单倍型的小鼠身上启动。其他实验 鉴定衣原体特异性识别抗原的多样性 CTL和CTL识别的抗原的多样性是否由 生殖器感染不同于腹膜内感染。第三， 我们建议确定这些抗原中的哪些可以激活CTL，哪些是 衣原体血清型之间既有保护性又有交叉反应。 该项目将通过以下方式为整个计划做出贡献 细胞和分子水平的抗原在一种 保护性CTL反应。该项目将补充项目3，其中 建议部分研究衣原体引起的CTL反应 人类的感染。该项目依赖于所提供的资源 由实验室(D)和统计(B)核心。通过提供新颖的 关于衣原体感染过程中产生的保护性CTL反应的信息 STD，该项目将为开发的总体目标做出贡献 一种预防和控制这些感染的疫苗。

Chlamydia is the most prevalent cause of bacterial sexually transmitted disease in the developed world, Causing both overt disease and infertility, and is also the leading cause of preventable blindness worldwide. Natural immunity against Chlamydia and the vaccine strategies that have been attempted to date provide protection against only limited serovars. The goal of these studies is to ascertain whether a vaccine strategy which primes a cytotoxic T-lymphocyte (CTL) response can provide protection against a wide range of Chlamydia serovars. We have cultured Chlamydia reactive CTL from infected mice and have shown that they are specific for and lyse Chlamydia infected cells in vitro. Upon adoptive transfer of these CTL into Chlamydia infected mice, a reduction in bacterial load can be measured in the spleen. The initial experiments in this proposal will determine whether CTL specific for Chlamydia can be primed in mice of different MHC haplotypes. Other experiments characterize the diversity of antigens recognized by Chlamydia specific CTL and whether the diversity of antigens recognized by CTL primed by genital infection differ from those primed intraperitoneally. Thirdly, we propose to determine which of these antigens can prime CTL which are both protective and cross-reactive between Chlamydia serovars. This project will contribute to the overall program by characterizing at the cellular and molecular level antigens which are important in a protective CTL response. The project will complement project 3 which proposes in part to examine the CTL response elicited by Chlamydia infection in humans. This project is dependent on the resources provided by the laboratory (D) and statistical (B) cores. By providing novel information about the protective CTL response engendered during Chlamydia STD, this project will contribute to the overall objective of developing a vaccine to prevent and control these infections.