GLASS-FIBER GEL MATRIX FOR PEPTIDE SYNTHESIS

描述：（改编自申请人的摘要） 对人类基因组进行测序， 人类蛋白质绘制结构特征--通常是短距离的 多肽-对受体和抗体识别以及其他 相互作用对于理解这些蛋白质的功能至关重要， 以及药物和疫苗的开发。为了满足日益增长的需求， 肽进行这些研究，新的方法和策略，肽 已经开发了合成方法，从组合文库， 含有数百万肽（皮摩尔水平）到多肽的混合物 合成器产生几十个肽（几十微摩尔）到大 比例合成器本文提出开发一种新的基质材料， 肽的快速合成水平（约1微摩尔）足以 允许对通过筛选发现的先导肽进行进一步的生物学测试 组合库这种材料是一种玻璃纤维支撑的凝胶， 允许在溶液和固相中合成用于测试的肽， 二维数组格式。预计玻璃纤维凝胶 材料将具有优于现有载体（膜， 薄膜、纸张等）在能力、稳定性和可获得性方面， 大分子（酶、抗体等）。 拟定商业应用： 从这项研究中开发的基质专注于用作肽合成的固体支持物，特别是限制性肽文库。其他应用包括核酸（DNA和RNA）和小分子组合文库的合成。 潜在产品 是基质，掺入96和384孔板中的基质，合成系统（基质 和相关仪器）和定制的肽、核酸或 有机小分子这些产品的市场利基是在基础研究，药物 发现、高通量筛选（HTS）和医学诊断。

DESCRIPTION: (Adapted from the applicant's abstract) The rapid progress towards sequencing the human genome has made available the sequences of thousands of human proteins. Mapping the structural features--typically short stretches of polypeptide--important for receptor and antibody recognition and other interactions is essential for understanding the function of these proteins, as well as for drug and vaccine development. To meet the escalating need for peptides to carry out these studies, new methods and strategies of peptide synthesis have been developed, ranging from combinatorial libraries which contain mixtures of millions of peptides (picomole level) to multipeptide synthesizers which produce dozens of peptides (tens of micromoles) to large scale synthesizers. It is proposed here to develop a new matrix material for the rapid synthesis of peptides at a level (around 1 micromole) sufficient to permit further biological testing of lead peptides discovered by screening combinatorial libraries. This material, a glass fiber-supported gel, will permit synthesis of peptides for testing in solution and in the solid-phase in a two-dimensional array format. It is expected that the glass fiber-gel material will have significant advantages over existing supports (membranes, films, paper, etc.) in terms of capacity, stability and accessibility to macromolecules (enzymes, antibodies, etc.). PROPOSED COMMERCIAL APPLICATION: The matrix developed from this research is focused on use as a solid-support for the synthesis of peptides, particularly restricted peptide libraries. Other applications include synthesis of nucleic acids (DNA and RNA) and small molecule combinatorial libraries. Potential products are the matrix, the matrix incorporated in 96 and 384 well plates, synthesis systems (matrix and associated instrumentation) and custom-made libraries of peptides, nucleic acids or small organic molecules. Market niches for these products are in basic research, drug discovery, high-throughput screening (HTS) and medical diagnostics.