SELECTING NEW CODING SEQUENCES BY PCR WITH CDNA PRIMERS

描述：(改编自申请人的摘要)目的 这一第二阶段小型企业技术转让项目是为了测试 聚合酶链式反应嵌合基因选择(CDS)方法在分离病毒中的应用 来自更大的人类基因组重叠群的未知编码序列。CDS 该方法基于两个同源DNA重组的原理。 因此，该方法将用于产生嵌合人EST-斑马鱼 在5‘端具有人EST序列的cDNA文库和 斑马鱼3‘端的同源基因。这些嵌合的cDNA可能是 用于在斑马鱼突变基因组中定位人类EST。另一个应用程序 CDS方法将产生一个人类看家的cDNA文库 这对鉴定组织特异性序列应该是有用的。 建议的商业应用：不可用

DESCRIPTION: (Adapted from the applicant's abstract) The purpose of this Phase II Small Business Technology Transfer project is to test the utility of a PCR chimeric cDNA selection (CDS) method for isolating unknown coding sequences from larger human genomic contigs. The CDS method is based on the principle of recombining two homologous DNAs. Thus the method will be used to generate chimeric human EST-zebrafish cDNA libraries having the human EST sequences at the 5'-ends and homologous zebrafish cDNA at the 3'-ends. These chimeric cDNAs could be used to map human ESTs in zebrafish mutant genomes. Another application of the CDS method will be to generate a human housekeeping cDNA library that should be useful in identifying tissue specific sequences. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE