MRNA EDITING PROTEINS--POSTTRANSCRIPTIONAL REGULATION OF HUMAN GENE EXPRESSION

我们的实验室专注于apoB mRNA的编辑，作为POST的模型 人类的转录碱基修饰。ApoB mRNA编辑是一个站点 胞苷残基在核苷酸上特异性脱氨基为尿苷 ApoB基因转录本中的6666位。脱氨基反应是 由胞苷脱氨酶介导的锌依赖过程 载脂蛋白B编辑组件1(APOBEC-1)，RNA编辑活性 APOBEC-1三次依赖于组装在一种酶复合体上 包括APOBEC-1(催化亚基)和辅助蛋白。在.期间 在过去的一年里，我们研究了apoB mRNA编辑的调节 转录后蛋白原磷酸化分析。

Our lab has focused on apoB mRNA editing as a model of post- transcriptional base modification in humans. ApoB mRNA editing is a site specific deamination of a cytidine residue to a uridine at nucleotide position 6666 in the ApoB mRNA transcript. The deaminination reaction is a zinc dependent process mediated by a cytadine deaminase referred to as apolipoprotein B editing component 1 (APOBEC-1), RNA editing activity of APOBEC-1 is trictly dependent on assembly on an enzyme complex that includes APOBEC-1(the catalytic subunit), and auxiliary proteins. During the past year, we have examined regulation of apoB mRNA editing by analysis of post-transcriptional proetin phosphorylation.