CORE--SIMIAN AND RODENT IMMUNOLOGY FACILITY

这一核心免疫学实验室的中和抗体成分将为该计划的所有三个项目提供支持。具体地说，我们将评估针对SIV、HIV-1和猿人免疫缺陷病毒(SIV)同源和异种毒株的中和抗体的大小、广度和持续时间。检测将酌情在人类CD_4+T细胞系或外周血单核细胞中进行，将包括T细胞系适应变异体和多个艾滋病毒-L初级分离株。这些评估将允许对项目I和项目2中分别描述的改良重组VRP疫苗载体和改良形式的Env产生的中和抗体进行详细评估。它们还将被设计用来评估中和抗体作为对抗实验SIV挑战的猕猴免疫的相关性，如项目3所述。项目I、2和3中引发的细胞免疫反应将在核心的细胞免疫学部分进行评估。CTL将被测量，细胞因子的合成将使用细胞内分析。此外，将使用四聚体染色，并为特定动物生产特定的四聚体。

The neutralizing antibody component of this core imrnunology laboratory will provide support for all three projects of this program. Specifically, we will assess the magnitude, breadth and duration of neutralizing antibodies against homologous and heterologous strains of SIV, HIV-1 and simian-human immunodeficiency virus (SHIV). Assays will be performed in either human CD4+ T cell lines or peripheral blood mononuclear cells (PBMC) as appropriate, and will include a T cell line adapted (TCLA) variant and multiple primary isolates of HIV- l. These assessments will permit a detailed evaluation of neutralizing antibodies generated by modified recombinant VRP vaccine vectors and modified forms of Env as described in Projects I and 2, respectively. They will also be designed to evaluate neutralizing antibodies as a correlate of immunity against experimental SIV challenge in macaques as specified in Project 3. Cellular immune responses elicited in Projects I, 2 and 3, will be assessed in the cellular immunology component of the core. CTL will be measured as will cytokine synthesis using intracellular assays. Further, tetramer staining will used and specific tetramers produced for particular animals.