Manufactured nanoparticle bioavailability and environmental exposure (nanoBEE)

nano-BEE联盟将利用经验数据开发和完善一个关键的模型子集，重点关注纳米材料的暴露及其在环境中的生物利用度。本研究的目的是（a）生成用于环境评估的受控和良好表征的NM库（B）证明软着陆的金簇提供合适的基准标记，以实现环境介质中NM的水生层析成像中的埃分辨率（c）证明NM环境改变过程可以通过聚集程度进行分类，溶解和表面改性，并通过实验和计算描述这些改性NM在环境分区之间的分配（d）开发NM生物利用度的修改生物动力学模型，反映水和食物暴露以及（e）验证NM对水生生物影响的生物配体模型。一个综合的计算和实验方案将使用电子显微镜，散射技术和光谱学检查制造的纳米材料的环境化学;使用可追踪的纳米材料来量化模型水生物种的流入和流出速率，包括在营养链中;并采用传统的毒理学终点措施以及最新的分子（“组学”）方法来量化生物效应以及确定新的机制的毒性。这些信息将输入到生物配体模型的NM类，输出预期的EC 50和其他结果的NM曝光和当地水化学的信息。通过与最终用户的接触，联盟将把NM身体负担和毒理学结果的预测与监管决策中有用的风险管理框架联系起来。

The nano-BEE consortia will develop and refine, using empirical data, a critical subset of models focused on exposure to nanomaterials (NMs) and their bioavailability in the environment. The objectives of this study are to (a) generate controlled and well-characterized NMs libraries for environmental assessment (b) prove that soft landed gold clusters provide suitable fiducial markers to enable angstrom resolution in aquatic tomography of NMs in environmental media (c) demonstrate that NM environmental modification processes can be classified by the extent of aggregation, dissolution and surface modification and to experimentally and computationally describe the partition of these modified NMs between environmental compartments (d) to develop modified biodynamic models for NM bioavailability that reflect both water and food exposures and (e) to validate biotic ligand models for NM effects on aquatic organisms. An integrated computational and experimental program will examine the environmental chemistry of manufactured NMs using electron microscopy, scattering techniques, and spectroscopy; use traceable NMs to quantify influx and efflux rates in model aquatic species, including in a trophic chain; and employ both conventional measures of toxicological endpoints as well as the latest molecular ('omics') methods to quantify biological effects as well as identify new mechanisms for toxicity. Such information will be input into biotic ligand models for NMs classes that output anticipated EC50 and other outcomes given information about NM exposure and local water chemistry. Through its engagement with end-users the consortia will link its predictions of NM body burdens and toxicological outcomes to risk management frameworks useful in regulatory decision-making.