GASTROINTESTINAL SIGNIFICANCE OF GASTRIN REGULATION

描述（改编自申请人的摘要）：这是一个建议， 继续研究胃泌素/CCK-B受体的性质和功能 和一种推定的Gly-延伸的胃泌素（G-Gly）受体。前一 资金期间，PI和他的合作者已经表明，Gly延长 中间体，酰胺化胃泌素的强制性前体，与 促胃液素并随生理刺激而分泌。甘氨酸延伸的 显示胃泌素通过单独的受体刺激细胞生长。的结合 胃泌素/CCK-B受体的结构和激活的结构要求 还研究了G蛋白。转录的几个关键组成部分 胃泌素/CCK-B受体基因的调节。本 该提案旨在扩大这些研究。以下具体目标将 被追求。1)阐明胃泌素/CCK-B的结构组分 在决定细胞生长和分泌中起重要作用的受体; 2） 表征胃泌素/CCK-B受体基因的结构组分， 在确定其表达和功能方面很重要; 3）检查 与Gly延伸的胃泌素介导的细胞生长相关的受体后事件; 和4）进一步表征Gly延伸的胃泌素的受体并克隆其 对应的cDNA。

DESCRIPTION (Adapted from the applicant's abstract): This is a proposal to continue studies on the properties and function of the gastrin/CCK-B receptor and a putative receptor for Gly-extended gastrin (G-Gly). During the previous funding period, the PI and his collaborators have shown that the Gly-extended intermediate, an obligatory precursor of amidated gastrin, is stored with gastrin and secreted with it in response to physiological stimuli. Gly-extended gastrin was shown to stimulate cell growth via a separate receptor. The binding of the gastrin/CCK-B receptor and the structural requirements for activation of G proteins were also studied. Several key components of transcriptional regulation of the gastrin/CCK-B receptor gene were identified. The present proposal is designed to extend these studies. The following specific aims will be pursued. 1) elucidate the structural components of the gastrin/CCK-B receptor that are important in determining cell growth and secretion; 2) characterize the structural components of the gastrin/CCK-B receptor gene that are important in determining its expression and function; 3) examine the post-receptor events linked to cell growth mediated by Gly-extended gastrin; and 4) further characterize the receptor for Gly-extended gastrin and clone its corresponding cDNA.