VASCULAR PATTERNING IN LEAVES OF ARABIDOPSIS THALIANA

我将研究PROV1在拟南芥叶片维管模式形成中的作用。叶片中的维管图案是由位置线索指定的，而发育中的叶脉本身很可能是叶片中随后的细胞类型图案的线索。最近利用增强子陷阱策略鉴定了PROV1基因，因为它在年轻的血管前细胞中特异表达，克隆时被发现编码一种受体样蛋白激酶。PROV1在维管指定和分化中的功能将通过在成熟植物中过度、低水平和有条件地表达PROV1来解决。在酵母双杂交系统中将通过异源表达来鉴定相互作用的蛋白，并将评估其活性，通过原位免疫定位确定亚细胞定位。对PROV1在叶脉发育中作用的研究以及对调控叶脉模式形成机制的深入研究，将对理解植物器官发生过程中的位置指定有价值的进展。

I will investigate the role of PROV1 in vascular pattern formation in leaves of Arabidopsis thaliana. Vascular patterning in leaves is specified by positional cues and the developing veins themselves likely act as cues for the subsequent patterning of cell types in the leaf. The gene PROV1 was recently identified, using an enhancer trap strategy, for its specific expression in young provascular cells and when cloned was found to encode a receptor-like-protein-kinase. The function of PROV1 in vascular specification and differentiation will be addressed by over-, under-, and conditionally expressing PROV1 in mature plants. The kinase activity will be evaluated, the subcellular localization will be determined by imunolocalization in situ, and interacting proteins will be identified using heterologous expression in the yeast two-hybrid system. The characterization of the role of PROV1 in vein development and insight into mechanisms which regulate vein pattern formation will result in valuable progress toward understanding positional specification during plant organogenesis.