CLONING AND CHARACTERIZATION OF AN IL-1-LIKE GENE

目的：从人卵巢cDNA文库中扩增出一个新的IL-1样基因（IL-1 T）。在对该基因进行表征后，将使用北方印迹分析和原位杂交研究分析IL-1 T的组织和细胞特异性表达。将产生融合蛋白用于抗体生产和用于使用免疫组织化学技术的蛋白质表达研究。此外，将生产重组蛋白并用于测定受体结合亲和力和生物活性的测定中。这项研究将确定这种IL-1样基因的功能，并有助于了解细胞因子在卵巢生理学中的潜在作用。

DESCRIPTION The proposed research will clone and characterize a novel IL-1 like gene (IL-1T), which was amplified from human ovary cDNA library. Following characterization of this gene, tissue-and cell-specific expression of the IL-1T will be analyzed using Northern blot analysis and in situ hybridization studies. A fusion protein will be generated for antibody production and for use in protein expression studies using immunohistochemical techniques. In addition, the recombinant protein will be produced and used in assays to determine receptor binding affinities and biological activities. The proposed study will identify the function(s) of this IL-1 like gene and assist in the understanding of the potential role(s) of cytokines in ovarian physiology.