GABA TRANSPORTERS OF RETINAL NEURONS AND GLIA

描述（改编自申请人的摘要）：氨基酸GABA起作用 在脊椎动物的视觉信息处理中起着重要作用 视网膜。 神经元和神经胶质细胞的载体介导的GABA转运是一个关键 在GABA能途径的正常运作的元素。 人们早就 怀疑中枢GABA的神经元和神经胶质转运系统 神经系统是不同的，具有不同的特性。 的目的 拟议的项目是研究结构和功能上的差异， 神经元和神经胶质GABA转运蛋白之间的关系。 电生理技术，包括全细胞和穿孔贴片 记录，将被用来检查GABA运输的性质， 酶促分离的水平（神经）和Muller（神经胶质）细胞， 冰鞋视网膜 此外，这些细胞的GABA转运蛋白将被 克隆，然后在卵母细胞中表达;双电极电压钳和巨噬细胞 来自卵母细胞的斑片记录将用于检查功能和 药理学特性 这些研究应阐明 神经元和神经胶质GABA转运机制在建立和 维持正常的视网膜功能。

DESCRIPTION (Adapted from applicant's abstract): The amino acid GABA plays an important role in the processing of visual information in the vertebrate retina. Carrier-mediated transport of GABA by neurons and glia is one key element in the proper functioning of GABAergic pathways. It has long been suspected that neuronal and glial transport systems for GABA in the central nervous system are distinct and have differing properties. The purpose of the proposed project is to examine structural and functional differences between neuronal and glial GABA transporters in the retina. Electrophysiological techniques, including whole- cell and perforated patch recordings, will be used to examine the properties of GABA transport in enzymatically isolated horizontal (neural) and Muller (glial) cells of the skate retina. In addition, the GABA transporters of these cells are to be cloned and then expressed in oocytes; dual-electrode voltage-clamp and giant patch recordings from oocytes will be used to examine functional and pharmacological properties. These studies should clarify the role that neuronal and glial GABA transport mechanisms play in establishing and maintaining normal retinal function.