SOMATIC CELLS AND SPERMATOCYTE MAINTENANCE IN DROSOPHILA

睾丸癌和男性不育症的发病率正在上升。 要深入了解这一现象的细胞原因，只有对精子发生的调控途径有系统的了解。 为了产生有功能的精子，在精子发生过程中协调了几种不同的细胞过程。 这些过程包括精母细胞的生长和成熟，精母细胞的生长和成熟与周围的体细胞密切相关，这表明体细胞-生殖系通讯参与其中。 这一提议的重点是果蝇的精子发生，这与哺乳动物的精子发生相似，遗传分析提供了一种系统地剖析精母细胞发育所需信号的方法。转录激活因子Eyes absent（Eya）在体细胞囊胞中表达，在那里它是维持包囊精母细胞所必需的。 Sine oculis（So）是一种同源结构域蛋白，似乎与Eya在转录调控复合物中起作用，也是精母细胞维持所必需的。 待检验的假设是精母细胞发育需要来自囊细胞的Eya/So依赖性信号。将评估Eya/So在囊肿细胞命运和行为中的作用。 此外，Eya和So影响精母细胞发育的阶段将被定义。 接下来，Eya和So的异位表达将测试Eya/So依赖性信号是许可的还是指导性的。 补充的方法，包括40精母细胞变性突变体的分析和抑制基因的筛选，将试图确定从体细胞的信号，或其在精母细胞中的响应组件。

The incidence of testicular cancers and of male infertility is increasing. Insight into the cellular causes of this can only come with a systematic understanding of the regulatory pathways governing spermatogenesis. In order to generate functional sperm, several diverse cellular processes are coordinated during spermatogenesis. These processes include the growth and maturation of spermatocytes, which occurs in close association with surrounding somatic cells, suggesting that soma-germline communication is involved. This proposal focuses on spermatogenesis in Drosophila, which is similar to that in mammals, and in which genetic analysis provides a way to systematically dissect the signals necessary for spermatocyte development. The transcriptional activator Eyes absent (Eya) is expressed in somatic cyst cells where it is required for the maintenance of encysted spermatocytes. Sine oculis (So), a homeodomain protein that appears to act in a transcriptional regulatory complex with Eya, is also required for spermatocyte maintenance. The hypothesis to be tested is that an Eya/So-dependent signal from cyst cells is required for spermatocyte development. The role of Eya/So in cyst cell fate and behavior will be assessed. In addition, the stage at which Eya and So affect spermatocyte development will be defined. Next, the ectopic expression of Eya and So will test whether the Eya/So-dependent signal is permissive or instructive. Complementary approaches, including the analysis of 40 spermatocyte degeneration mutants and a genetic screen for suppressors, will attempt to identify the signal from somatic cells, or components of its response in spermatocytes.