DIETARY FAT AND THE OXIDATION OF PLAQUE LIPIDS

描述：拟定的研究将调查脂肪酸 类型和维生素E浓度调节：（1）脂质的量 从动脉粥样硬化斑块分离的氧化产物，和（2） 导致脂质氧化产物形成的机制。 NZW 兔子将维持在富含饱和，单不饱和 或n-6多不饱和脂肪。 饮食将包含高或低 维生素E的浓度将使用GC-MS表征脂质 氧化产物，并推断导致氧化的机制 假设油酸、3-硝基酪氨酸的单过氧化产物 和3-氯酪氨酸反映了过氧亚硝酸根氧化的起始， 游离过渡金属或髓过氧化物酶。

DESCRIPTION: The proposed studies will investigate whether fatty acid type and vitamin E concentrations modulate: (1) the amounts of lipid oxidation products isolated from atherosclerotic plaques, and (2) the mechanisms that lead to the formation of lipid oxidation products. NZW rabbits will be maintained on diets enriched in saturated, monounsaturated or n-6 polyunsaturated fat. The diets will contain either high or low concentrations of vitamin E. GC-MS will be used to characterize lipid oxidation products and to infer the mechanisms leading to oxidation assuming that mono-peroxidation products of oleic acid, 3-nitrotyrosine and 3-chlorotyrosine reflect initiation of oxidation by peroxynitrite, free transition metals or Myeloperoxidase, respectively.