OXYGEN MEASUREMENTS IN VIVO BY PHOSPHORESCENCE QUENCHING

描述：调查人员提出了一项计划，以开发可靠的 基于磷光猝灭法的氧气测定方法 活体动物体内浓度和人体浅表组织体积 患者，如其摘要中进一步描述的： “氧依赖的磷光猝灭是一种非侵入性的、高度 精确、光学的氧气测量方法，包括在条件下 它要么没有其他合适的方法，要么现有的方法 是不够的。这是生物医学急需的新技术。 研究并作为临床诊断工具，因为它可以在非 生理状态下组织氧合的有创、实时测量 条件和实时的。为了将这项新技术带到它的 充分发挥潜力，并使最大数量的用户能够访问它，我们 将：1.设计和合成水溶性、无毒的荧光粉 在组织的近红外“窗口”中吸收和磷化。一个。 确定磷光体微环境在氧猝灭中的作用 过程，例如，氧气对磷光体的访问，对pH的依赖和 温度、光化学稳定性等；B.测定化学成分 控制荧光粉通过血管的渗透性的特性 管壁(渗入血管内腔)和肾脏的排泄。 2.开发新的、高性能的频域磷度计 在活体内测量氧压。3.发展数学形式主义 提取氧分布(直方图)所需的 测量磷光的微血管成像计算分析 腐烂。4.建造和测试能够提供3D的新光学系统 组织中相对缺氧区的毫米分辨率地图 每一面有几厘米的体积。 “潜在的临床应用包括诊断和临床评估。 糖尿病、周围血管疾病、脑血管并发症 和心血管事件以及检测和治疗设计 肿瘤的治疗。“

DESCRIPTION: The investigators propose a program to develop reliable methods based on phosphorescence quenching for the measurement of oxygen concentrations in living animals and superficial tissue volumes in human patients, as further described by their abstract: "Oxygen dependent quenching of phosphorescence is a non invasive, highly accurate, optical method for measuring oxygen, including under conditions for which there are either no other suitable method or the existing methods are inadequate. This is new technology which is badly needed in biomedical research and as a diagnostic tool in the clinics, because it can be used non invasive, real time, measurements of tissue oxygenation under physiological conditions and in real time. In order to bring this new technology to its full potential and to make it accessible to a maximum number of users, we will: 1. Design and synthesize water soluble, non-toxic phosphors which absorb and phosphoresce in the near infra red 'window' of tissue. A. Determine the role of the phosphor microenvironment on the oxygen quenching process, e.g. access of oxygen to the phosphor, dependence on pH and temperature, photochemical stability, etc.; B. Determine the chemical properties which control permeability of the phosphors through the vascular wall (leakage into the intravascular space) and of excretion by the kidney. 2. Develop new, high performance, frequency domain phosphorometers for measuring oxygen pressure in vivo. 3. Develop the mathematical formalism needed for extraction of oxygen distributions (histograms) for the microvasculature by computational analysis of the measured phosphorescence decays. 4. Construct and test new optical systems capable of providing 3D maps, at millimeter resolution, of regions of relative hypoxia in tissue volumes several centimeters on a side. "Potential clinical applications include diagnosis and clinical evaluation of complications of diabetes, peripheral vascular disease, cerebrovascular and cardiovascular events as well as detection and therapy design for treatment of tumors."