CORE--PHYSIOLOGY

生理学核心将提供心肌组织样本， 线粒体供各个项目使用。这些样品 从基线对照中的分离的缓冲液灌注心脏获得 缺血后和缺血再灌注期间的状态。使用 一个共同的核心设施，执行隔离缓冲液灌注心脏 制备和分离心肌线粒体将确保所有 项目使用可比较的实验制剂。芯会 促进项目之间的信息交流，因为 实验心脏模型、灌注技术和线粒体的方法 在每个项目中都使用了隔离。这种方法将加强 应用个别项目取得的实验结果， 其他项目感兴趣的实验问题。的使用 核心也将增加项目之间的组织共享将减少 适当使用动物，优化整体计划效率。的 核心还将确保个人的隔离心脏准备和 线粒体分离物符合预定义的控制标准， 在此模型中的先前实验工作。核心将解释并使 将离体心脏实验的结果提供给每一个 通过核心维护数据库和全方案网络向调查员提供信息。 分离的线粒体和心脏组织样本将被编入一个 公共数据库沿着有离体心脏的生理结果 实验将储存和维护数据结果和组织样本 由核心，并将一起发布给个别研究者， 需要，允许每个项目轻松整合生物化学发现 相关的生理结果

The Physiology Core will provide samples of myocardial tissue and isolated mitochondria for use by the individual projects. These samples are obtained from isolated, buffer perfused hearts in the baseline control state following ischemia and during ischemia and reperfusion. The use of a common core facility that performs isolated buffer-perfused heart preparations and isolates cardiac mitochondria will ensure that all projects utilize comparable experimental preparations. The core will facilitate the exchange of information among projects, since a common experimental heart model, perfusion technique, and method of mitochondrial isolation is used in each project. This approach will enhance the application of experimental results obtained by an individual project to experimental questions of interests to the other projects. The use of a core will also increase the sharing of tissue among projects will decrease animal use where appropriate, optimizing overall program efficiency. The core will also ensure that individual isolated heart preparations and mitochondrial isolates meet predefined control standards based upon our previous experimental work in this model. The core will interpret and make available the results of the isolated heart experiments to each investigator via a core-maintained database and program-wide network. Isolated mitochondria and heart tissue samples will be catalogued into a common database along with the physiologic results of the isolated heart experiments. Data results and tissue samples will be stored and maintained by the core, and will be released together to individual investigators as needed, allowing each project to easily integrate biochemical findings with pertinent physiologic results.