CORE--PHYSIOLOGY

生理学核心将提供心肌组织样本和分离的 供个别项目使用的线粒体。这些样品是 从基线对照中的隔离、缓冲液灌流的心脏获得 缺血后及缺血和再灌流期间的状态。对.的使用 执行隔离缓冲灌流心脏的公共核心设备 制备和分离心肌线粒体将确保所有 项目使用可比的实验准备。核心将会 促进项目之间的信息交流，因为共同的 线粒体实验性心脏模型、灌流技术及方法 每个项目都使用隔离。这种方法将增强 单个项目的实验结果应用于 其他项目感兴趣的试验性问题。的用法 核心还将增加项目之间的组织共享将减少 在适当的情况下使用动物，优化整体程序效率。这个 CORE还将确保个人隔离心脏制剂和 线粒体分离株符合预定义的控制标准 以前在这个模型中的实验工作。核心将解释和制作 将离体心实验的结果提供给每个人 调查员通过核心维护的数据库和整个方案的网络。 分离的线粒体和心脏组织样本将被编目到一个 公共数据库和离体心的生理学结果 实验。数据结果和组织样本将被存储和维护 ，并将作为个人调查人员一起释放 需要，允许每个项目轻松地整合生化研究结果 有相关的生理结果。

The Physiology Core will provide samples of myocardial tissue and isolated mitochondria for use by the individual projects. These samples are obtained from isolated, buffer perfused hearts in the baseline control state following ischemia and during ischemia and reperfusion. The use of a common core facility that performs isolated buffer-perfused heart preparations and isolates cardiac mitochondria will ensure that all projects utilize comparable experimental preparations. The core will facilitate the exchange of information among projects, since a common experimental heart model, perfusion technique, and method of mitochondrial isolation is used in each project. This approach will enhance the application of experimental results obtained by an individual project to experimental questions of interests to the other projects. The use of a core will also increase the sharing of tissue among projects will decrease animal use where appropriate, optimizing overall program efficiency. The core will also ensure that individual isolated heart preparations and mitochondrial isolates meet predefined control standards based upon our previous experimental work in this model. The core will interpret and make available the results of the isolated heart experiments to each investigator via a core-maintained database and program-wide network. Isolated mitochondria and heart tissue samples will be catalogued into a common database along with the physiologic results of the isolated heart experiments. Data results and tissue samples will be stored and maintained by the core, and will be released together to individual investigators as needed, allowing each project to easily integrate biochemical findings with pertinent physiologic results.