CYTOSKELETAL CELL SURFACE INTERACTION IN ORAL EPITHELIAL CELLS

这项研究的目的是确定基本的 调节细胞骨架之间相互作用的机制 角蛋白中间丝和细胞表面相关 牙龈粘附连接、桥粒和半桥粒 上皮细胞 拟议研究的长期目标是 深入了解上皮细胞的分子基础 牙周病的一个标志性的迁移。 的 具体目标包括确定中间灯丝如何 相关蛋白IFAP 300连接桥粒， 半桥粒到中间花丝。 为此，IFAP300 将被克隆并测序一旦完整的cDNA序列 瞬时转染将用于确定 这种重要的跨桥蛋白的生理功能。 其他目的是确定在体外的分子相互作用 在角蛋白、IFAP 300和主要胞浆斑块中， 桥粒蛋白（桥粒斑蛋白）和半桥粒蛋白 （BP 230）。 体内研究的目标是确定 活细胞中角蛋白中间丝的动力学性质 使用显微成像技术。

The objectives of this research are to determine the basic mechanisms that regulate the interactions between cytoskeletal keratin intermediate filaments and the cell surface associated adhesion junctions, desmosomes and hemidesmosomes, of gingival epithelia cells. The long term goal of the proposed studies is to gain insights into the molecular bases of the epithelial cell migration that represents a hallmark of periodontal disease. The specific aims include determining how the intermediate filament associated protein, IFAP300, connects desmosomes and hemidesmosomes to intermediate filaments. To this end, IFAP300 will be cloned and sequenced. Once the complete cDNA sequence is available, transient transfection will be used to determine the physiological functions of this important cross-bridging protein. Other aims are to determine in vitro the molecular interactions among keratins, IFAP300 and the major cytoplasmic plaque proteins of the desmosome (desmoplakin) and the hemidesmosome (BP230). In vivo studies will be targeted at determining the dynamic properties of keratin-intermediate filaments in living cells using microscopic imaging techniques.