LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

液相色谱（LC）结合质谱（MS）检测目前无法在韦恩州立大学使用。申请人非常需要这种分析工具来进行各种正在进行的项目，包括表征和鉴定，以及鉴定：修饰的DNA和RNA组分，药物代谢物和化学合成产物。该应用程序列出了六个主要用户，他们都是由NIH资助的。LC-MS仪器将提供更灵敏的分析，更不容易形成伪影，更少的时间消耗或不可能与目前的仪器。我们要求的LC- MS仪器将由韦恩州立大学的中央仪器设施安置和维护。该设施从许多内部和外部来源获得资金，确保LC-MS长期得到适当的护理。中央仪器设施还提供色谱和质谱技术方面的博士级专业知识，并为共享仪器使用制定了完善的程序。总之，我们机构对LC-MS的需求至少有六个nih资助的用户，我们有能力在其使用寿命期间操作和维护该仪器。

Liquid chromatography (LC) combined with mass spectral (MS) detection is currently not available at Wayne State University. This analytical tool is very much needed by the applicants for a variety of ongoing projects involving characterization and identification and identification of: modified DNA and RNA components, drug metabolites and products of chemical syntheses. The application lists six major users who are all funded by the NIH. The LC-MS instrument would provide for analyses that are either more sensitive, less prone to artefact formation, much less time consuming or not possible with the current instrumentation. The LC- MS instrument that we request will be housed and maintained by the Central Instrument Facility of Wayne State University. This facility has funding from a number of internal and external sources, assuring proper care of the LC-MS over the long term. The Central Instrument Facility also provides Ph.D.-level expertise in chromatographic and mass spectral techniques and has well-developed procedures for shared instrument use. In summary, the need for LC-MS at our institution is clearly present for at least six NIH-funded users and we have the ability to operate and maintain this instrument throughout its useful life.