ACQUISITION OF MASS SPECTROMETER FOR CORE FACILITY

请求提供资金，用于购买基质辅助激光解吸/电离飞行时间质谱仪的80%费用。该仪器的5个主要用户和8个次要用户将来自威斯康星大学麦迪逊分校的生物化学系。其他用户将来自该大学约750名从事生物医学研究的教员的实验室。目前，威斯康星大学麦迪逊分校只有一台MALDI-TOF质谱仪。该仪器已被其他用户饱和，位于1/4英里外的化学系，主要用户和次要用户不容易访问。所要求的仪器的13个主要和次要用户是NIH资助的研究生物分子及其相互作用的研究人员。他们有积极的合作，定期分享设备和想法，并参加共同的培训和学位课程。仪器的主要用户将是在主要用户实验室工作的90名学生和工作人员。MALDI-TOF质谱仪将被放置在现有的校园生物物理仪器设施，这是位于生物化学系。在那里，质谱仪将加入其他最先进的仪器，用于表征生物分子及其相互作用。该设施由一名全职仪器专家管理，他将监督质谱仪的日常使用和维护。预计学生和工作人员将获得自己的MALDI-TOF质谱后，从仪器专家的短期培训课程。

Funds are requested for 80% of the cost of purchasing a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The 5 primary users and 8 secondary users of this instrument would be from the Department of Biochemistry at the University of Wisconsin-Madison. Other users would be from laboratories of the approximately 750 faculty members performing biomedical research at the university. Currently, the University of Wisconsin-Madison campus has only one MALDI-TOF mass spectrometer. This instrument is saturated with other users and, located 1/4-mile away in the chemistry department, is not readily accessible to the primary and secondary users. The 13 primary and secondary users of the requested instrumentation are NIH-funded investigators who study biomolecules and their interactions. They have active collaborations, routinely share equipment and ideas, and participate in common training and degree programs. The major users of the instrumentation would be the 90 students and staff working in the laboratories of the primary users. The MALDI-TOF mass spectrometer would be placed in the existing campus Biophysics Instrumentation Facility, which is located in the Department of Biochemistry. There, the mass spectrometer would join other state-of- the-art instruments for characterizing biomolecules and their interactions. This facility is managed by a full-time Instrumentation specialist, who would oversee the day-to-day use and maintenance of the mass spectrometer. It is anticipated that students and staff would obtain their own MALDI-TOF mass spectra after a short training session from the Instrumentation Specialist.