ACQUISITION OF A 400 MHZ NMR INSTRUMENT

快速的样品吞吐量对于位于中央核心设施的多用户科学仪器至关重要，例如麻省理工学院化学系的仪器设施(DCIF)。向国家卫生研究院申请资金，以协助购买一台完整的400兆赫核磁共振(核磁共振)仪器，以更好地为这项提案的七个主要用户组的104名研究人员提供服务。所要求的仪器将取代已有13年历史的瓦里安XL-300仪器(它最近经历了相当长的停机时间，包括磁铁猝灭)，并将成为从事NIH资助的一系列研究项目的研究人员的主要主力仪器。在麻省理工学院的DCIF中包括一台现代化的400兆赫核磁共振仪器是一项重大倡议的组成部分--包括最近耗资54万美元对DCIF进行的翻修--以升级和更新向系和其他学术研究人员提供的仪器服务。提高了效率，加强了系内与生物学有关的研究。将受益于该仪器的项目包括：不对称催化、新反应开发、全合成、电子转移研究、低聚糖合成和多肽构象研究。目前，DCIF的所有核磁共振仪器都严重过时，只有一台除外。即使是DCIF最新的核磁共振仪器(Unity300，带有用于固态工作的宽带磁铁)也已经有八年的历史了。除了布鲁克AC-250以外，所有其他核磁共振仪器都有十多年的历史了，只有九年的历史。DCIF缺乏现代化和高效的核磁共振仪器，对生产率产生了负面影响。NIH资助的对中小分子的研究将大大促进一个400 MHz的核磁共振仪器，具有更高的灵敏度，移动数据的互联网接入，自动样品更换，快速自动匀场能力，以及四核探针。

Rapid sample throughput is essential for multi-user scientific instruments located in central core facilities such as the Department of Chemistry's Instrumentation Facility (DCIF) at the Massachusetts Institute of Technology (MIT). Funds are requested from the NIH to assist in the purchase of a complete 400 MHz nuclear magnetic resonance (NMR) instrument to better serve 104 researchers from the groups of this proposal's seven Major Users. The requested instrument will replace a 13-year-old Varian XL-300 instrument (which has recently experienced considerable down time including a magnet quench) and will sere as the primary workhorse instrument for researchers working on a wide array of NIH-funded research programs. The inclusion of a modern 400 MHz NMR instrument in the DCIF at MIT is an integral part of a major initiative- -including the recent $540,000 renovation of the DCIF--to upgrade and modernize the instrument services provided to Departmental and other academic researchers. The increased efficiency, enhanced biologically- related research in the Department. Projects that will benefit from the acquisition of this instrument include the following: asymmetric catalysis, new reaction development, total synthesis, electron transfer studies, oligosaccharide synthesis, and investigations of peptide conformation. At present, all but one of the DCIF's NMR instruments is severely outdated. Even the DCIF's newest NMR instrument (a Unity 300 with a widebore magnet intended for solid-state work) is eight year old. All other NMR instruments are more than ten years old excepting a Bruker AC-250 which is nine years old. The lack of modern and efficient NMR instrumentation in the DCIF negatively impacts productivity. NIH-funded research on small and intermediate size molecules being carried out in the Department would be greatly facilitated by a 400 MHz NMR instrument with increased sensitivity, internet access for moving data, an automatic sample change, rapid autoshimming capability, and a four- nucleus probe.