600MHZ WIDE BORE NMR SPECTROMETER

该拨款提案要求为600兆赫宽口径核磁共振光谱仪提供部分资金支持。该仪器将位于核磁共振研究中心，作为整合生物医学专用核磁共振仪器和促进合作研究的总体战略的一部分。所要求的光谱仪将满足现有仪器无法满足的主要用户群体对体内微成像和光谱学日益增长的需求。确定了一个由八个独立研究项目组成的主要用户组，该组的成员在获得拨款时已经或期望得到同行认可的NIH支持。在提案的研究计划的介绍部分以及在个别研究项目描述中讨论了所提议的光谱仪的理由和利用。提案的主要力量来自于个人研究项目本身，所有这些都与临床和生物医学相关。阿尔茨海默病将利用转基因小鼠模型（Caserta）和结构肽分析（kraft）进行机制研究，这两种方法是互补的。两个项目（Groothuis和Wyrwicz）研究了中枢神经系统内的药物传递和药代动力学。Lurie综合癌症中心的合作者有研究乳腺癌（Thor）的致癌机制的项目，结肠癌（Knop）治疗失败的原因的项目，以及肿瘤血管生成领域的两个主要贡献者的项目（Bouck和Soff）。西北大学家族在神经科学和癌症领域的广泛代表性是芝加哥大都会地区任何其他科学联盟所无法比拟的。该提案的另一个优势是通过核磁共振研究中心提供的多个专业的专业知识，并得到成功实施实验策略所需资源的支持。

This grant proposal requests partial funding support for a 600 MHz wide bore NMR spectrometer. The instrument will be located at the Center for NMR Research as part of an overall strategy to consolidate biomedically dedicated NMR instruments and promote collaborative research. The requested spectrometer will accommodate the growing needs of a major user group for in vivo microimaging and spectroscopy that cannot be met with the existing instrumentation. A major user group comprising eight separate research projects is identified, and members of the group have, or expect to have at the time of the grant award, peer-received NIH support. Justification and utilization of the proposed spectrometer are discussed in the introductory section of the research plan of the proposal, as well as within the individual research project description. The major strength of the proposal comes from the individual research projects themselves, all with clinical and biomedical relevance. Alzheimer's disease will be investigate mechanistically using both transgenic mouse models (Caserta) and structural peptide analysis (Krafft) which are complementary. Drug delivery and pharmacokinetics within the central nervous system are addressed in two projects (Groothuis and Wyrwicz). The Lurie Comprehensive Cancer Center collaborators have projects which investigate the carcinogenesis of breast cancer (Thor), causes of treatment failures in colon cancer (Knop) and two projects from major contributors to the field of tumor angiogenesis (Bouck and Soff). This broad representation within the Northwestern University Family focused on the areas of neuroscience and cancer is unmatched by any other scientific consortium in the Chicago metropolitan area. An additional strength of the proposal is the expertise of the multiple specialties available through the Center for NMR Research, and supported with resources necessary for successful implementation of experimental strategies.