TRANSMISSION ELECTRON MICROSCOPE--PHILIPS CM120

由14名调查人员组成的小组要求资金购买高价 计算机控制的Phillips CM120传输电子分辨率 显微镜(TEM)。其中13名用户目前拥有NIH支持，1名用户 得到了NSF/海军的支持。该组织提议取代目前唯一的 细胞生物学与解剖学系内可用的透射电子显微镜。这个 15岁的JEOL 200CX在 过去五年，缺少许多关键配件，并有一些标准 不适合研究生物标本的特征。 例如，200 CX专为在高分辨率下进行低对比度操作而设计 加速电压高达200千伏，这为 研究厚厚的标本。例如，200 CX是为Low设计的 在200千伏以上的高加速电压下的对比运行， 为研究厚标本提供了一些有利条件。例如， 200 CX专为高速加速下的低对比度操作而设计 高达200千伏的电压，这为研究厚度提供了一些优势 标本。年，对此功能的需求已显著减少 近年来，更重要的是，该设计损害了运营和 低放大倍率和标准电压。此外，对瞬变电磁的需求 在过去五年中有所增加，对 200CX的调度和性能。所请求的仪器将 用现有的TM克服当前的许多问题，并提供 提高效率和图像质量的关键功能 制作。此外，所要求的配件将为该团队提供 进行新型实验的能力目前是不可能的。 许多学生要求对样本进行相对较低的检查 固有的对比度，如带有金抗体复合体的标本。这个 菲利普斯CM120的专利双物镜 设计用于在低倍数下检查生物标本 对比度高，视野开阔。高分辨率底座 Ccd数码相机附件将允许收集数字化图像 用于在线傅立叶分析、计算机断层扫描、自动 剪辑、低/最小剂量成像和出版物的制作 高质量的胶片，因此在许多应用中取代了传统胶片。 这些功能和其他功能非常适合于多学科 需要瞬变电磁的项目。这一工具将大大增强 一大群经验丰富的科学家的生产力，其中9人来自 细胞生物与解剖学系和其他系的5名学生 大学，并显著改善了 社区。

A group of fourteen investigators requests funds to purchase a high resolution, computer controlled Phillips CM120 transmission electron microscope (TEM). Thirteen of the users have current NIH support and one has NSF/Navy support. The group proposes to replace the only currently available TEM within the Department of Cell Biology & Anatomy. The fifteen-year-old JEOL 200CX has had progressively more downtime in the last five years, lack many key accessories, and has some standard features that are not well-suited for studying biological specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltages up to 200 kV, which offers some advantages for studying thick specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltage up to 200 kV, which offers some advantages for studying thick specimens. For example, the 200 CX was designed for low contrast operation at high accelerating voltages up to 200 kV, which offers some advantages for studying thick specimens. The demand for this feature has decreased significantly in recent years and, importantly, the design has compromised operation and low magnification and standard voltages. Moreover, the need for the TEM has increased over the last five years, placing further demands on scheduling and performance of the 200CX. The requested instrument would overcome many of the current problems with the existing TEM and provide key features to improve efficiency and the quality of the images produced. Furthermore, the requested accessories would give the group the ability to perform new types of experiments not currently possible. Many students require the examination of specimens with relatively low inherent contrast, such as specimens with gold-antibody complexes. The patented twin objective lens of the Phillips CM120 is especially designed for examining biological specimens at low magnifications with high contrast and a wide field of view. The high resolution bottom mount CCD digital camera accessory would permit collection of digitized images for on-line Fourier analysis, computerized tomography, automatic montaging, low/minimum dose imaging and production of publication quality prints, thus replacing conventional films for many applications. These and other features are ideally suited for the multi-disciplinary projects requiring TEM. This instrument would greatly enhanced the productivity of the large group of experienced scientists, nine from the Department of Cell Biology & Anatomy and five from other departments at the University, and significantly improve the microscopic facilities in the community.