NORAN OZ CONFOCAL LASER SCANNING SYSTEM

奥尔巴尼医学院（AMC）的一组研究人员正在申请资金，以购买Noran OZ共聚焦激光扫描显微镜（CLSM），这是一种之前无法在AMC使用的仪器。Drs。McKeown-Longo、LaFlamme、Higgins、Mazurkiewicz和Saba正在研究细胞-细胞和细胞-基质的相互作用。这些研究集中在各种信号分子、整合素、细胞连接、细胞骨架和基质蛋白上，并使用免疫荧光和CLSM来定位这些分子或细胞结构元件，以记录它们在整合素-细胞骨架-基质蛋白上的分布，以及它们彼此之间的关系，细胞连接（紧密连接、粘附连接和半桥粒）或细胞-基质相互作用，随后是各种刺激、发育线索、或者基因转染。赖斯博士正在分析小鼠和大鼠须垫发育过程中皮肤末梢复杂的三维组织中的荧光标记结构和分子。Black博士使用荧光长链脂肪酸类似物BODIPY-3823来评估Fat1p对脂肪酸的吸收，Fat1p是酵母质膜中发现的一种膜结合的长链脂肪酸运输蛋白。他正在研究delta - 1基因突变对脂肪酸运输的影响。施耐德博士正在测试肾上腺染色质细胞分泌的一个新假设，该假设涉及存在于分泌颗粒膜中的钠钙交换蛋白，并在胞吐过程中插入质膜。坦普尔博士在研究大鼠皮层神经元原代培养中的分化时，检查了细胞中各种与钙离子通道相关的受体的外观和活性。两位研究人员都在用msec动力学监测细胞质[Ca++]的生理反应，这需要Noran的快速扫描能力，Melendez博士也在研究MnSOD过表达对活培养的致瘤细胞和非致瘤细胞氧化状态的影响。Drs。Gosselin和Mazurkiewicz研究了GFP标记的MHC II类（MHC II）和外源源抗原在培养的活人单核细胞抗原呈递中的运输。这组研究将充分利用Noran OZ CLSM的能力。

A group of investigators at the Albany Medical College (AMC) are requesting funds to purchase a Noran OZ Confocal Laser Scanning Microscope (CLSM), a type of instrument heretofore unavailable for their use at the AMC. Drs. McKeown-Longo, LaFlamme, Higgins, Mazurkiewicz, and Saba, are studying cell-cell and cell-matrix interactions. The studies focus on various signaling molecules, integrins, cell junctions, cytoskeleton, and matrix proteins and the use of immunofluorescence and the CLSM to localize these molecules or elements of the cell structures to note their distribution with relationship to one another, to cell junctions (tight junctions, adherens junctions, and hemi-desmosomes) or cell-matrix interactions at integrin-cytoskeleton-matrix proteins, subsequent to a variety of stimuli, developmental cues, or gene transfections. Dr. Rice is analyzing fluorescently labelled structures and molecules in the complex three-dimensional organization of the cutaneous endings during development of the whisker pads of mice and rats. Dr. Black uses the fluorescent long-chain fatty acid analog, BODIPY-3823, to assess the uptake of fatty acids by Fat1p, a membrane- bound long-chain fatty acid transport protein found in yeast plasma membranes. He is studying the effects of mutations in the gene, deltafat1, on fatty acid transport. Dr. Schneider is testing a novel hypothesis of secretion in adrenal chromaffin cells that involves a Na- Ca exchange protein that is present in secretory granule membranes and that is inserted into the plasma membrane during exocytosis. Dr. Temple, in studying differentiation in primary cultures of rat cortical neurons, examines the appearance and activity of various receptor linked Ca++ channels in her cells. Both researchers are monitoring physiologic responses in cytoplasmic [Ca++] with msec kinetics that require the fast-scan capabilities of the Noran, as does Dr. Melendez who is studying the effect of MnSOD overexpression on the oxidant status of tumorigenic and non-tumorigenic cells in live cultures. Drs. Gosselin and Mazurkiewicz study the trafficking of GFP tagged-MHC Class II (MHC II) and exogenously derived antigens in antigen presentation in live human monocytes in culture. This group of researches will fully utilize the capabilities of the Noran OZ CLSM.