BIACORE 2000 SYSTEM

这是RR 12924的修订申请，最初于1997年3月20日提交。 我们感谢评审员指出了优势（“编写良好且强有力的组织计划”）和弱点（“10名研究者的仪器时间不足，某些项目缺乏获得BIAcore 2000的理由”）。 我们已就这些批评作出明确回应，并将其纳入经修订的申请中。 我们已经将合作研究者的数量从10人减少到6人，重点关注在其研究项目中最需要BIAcore 2000的研究者。 这一变更显然将为每位研究者留出充足的仪器时间。 此外，由于BIAcore 2000具有自动进样器功能，可由操作员预编程，因此许多样品测试可在夜间进行。 我们将充分利用BIAcore 2000的这一特点。 BIAcore 2000的任何剩余可用时间将分配给有适当需求的其他相关研究者。 正如本申请的正文所示，我们已经提供了关于BIAcore 2000的最佳和公平使用的更多细节。 为了解决评审员的第二个问题，每个合作研究者都提供了一个简短但更完整的背景，她/他的研究兴趣和更详细的BIAcore 2000将是必要的，他们的研究。 我们相信，随着这些变化，以及目前在整个辛辛那提地区没有此类工具的事实，审查人员将积极看待这一修订后的申请。

This is a revised application of RR12924, initially submitted on March 20, 1997. We thank the reviewer for pointing out the strengths ("well- written and strong organizational plan") and weaknesses ("insufficient availability of instrument time to 10 investigators and lack of justification in some projects to acquire BIAcore 2000"). We have clearly responded to the criticisms and incorporated them in this amended application. We have reduced the number of co-investigators from 10 to 6 focusing on investigators with the greatest need for the BIAcore 2000 in their research projects. This change will clearly allow sufficient instrument time for each investigator. Furthermore, since BIAcore 2000 has an autosampler function that can be pre-programmed by the operator, many of the sample tests can be performed overnight. We will exploit this feature of BIAcore 2000 to our advantage. Any remaining available time on BIAcore 2000 will be allotted to other interested investigators with appropriate needs. As is manifest in the body of this application, we have presented greater detail regarding the optimal and equitable usage of BIAcore 2000. To address the second concern of the reviewers, each co-investigator has provided a brief but more complete background of her/his research interests and a greater detail of how BIAcore 2000 will be necessary for their research. We trust that with these changes and the fact that currently no such instrument is available in the entire Cincinnati area, the reviewers will view this amended application favorably.