CORE--MORPHOLOGY

形态学核心的主要目标是通过促进皮肤样本显微分析技术的使用，提高新的P&F研究人员和现有皮肤研究人员的多功能性和生产力。这将通过维持已证实的试剂（抗血清）库，执行免疫染色方案，筛选新的抗血清，指导/优化原位杂交方案来完成，筛选转基因小鼠的组织学皮肤异常，并提供皮肤病理学协助。这个核心将提供一个开拓/培育的环境，在这里，在一个经验丰富的团队的指导下，最前沿的显微技术将被初步尝试和实现。该中心将实施引入具有更高灵敏度的新技术，并为更大的SDRC用户群体产生一系列途径特异性技术（整合素面板、细胞凋亡标记物、信号转导探针和抗血清），并加速从基于同位素的方案向非同位素方法的转变。Core将为显微镜标本提供最先进的包埋、切片、染色、定量形态测定、照相布局和打印，并提供及时和低成本的基础。形态学核心的另一个主要功能将是获得具有准确人口统计学和诊断数据的临床衍生人类样本。大量的、集中的皮肤病理和伤口愈合样本库的持续可用性是开展皮肤相关项目的研究人员的关键组成部分和宝贵资源。主任和所有研究助理将为P&F研究人员、SDRC资助研究人员及其博士后、研究生和实验室人员提供皮肤分析有用的显微技术方面的教学协助和建议。当冷冻组织被运送到分子遗传学中心或动物样本和/或从表型中心产生培养细胞时，分子遗传学中心和表型中心之间的合作互动将每天发生。

The primary objective of the Morphology Core is to enhance the versatility and productivity of both new P&F investigators and established skin investigators by facilitating access to techniques directed at the microscopic analysis of skin samples. This will be accomplished by maintaining banks of proven reagents (antisera), executing immunostaining protocols, screening new antisera, instructing/optimizing in situ hybridization protocols screening transgenic mice for histologic skin abnormalities, and providing dermatopathologic assistance. This core will provide a pioneering/nurturing environment where cutting edge microscopic techniques are initially tried and achieved under the guidance of an experienced team. The core will implement the introduction of new techniques with greater sensitivities and generate clusters of pathway- specific techniques (integrin panels, apoptosis markers, signals transduction probes and antisera) to the greater SDRC user group, and hasten the conversion from isotopic based protocols to non-isotopic methods. The Core will render state-of-the-art embedding, sectioning, staining, quantitative morphometry, photographic layout and printing for microscopic specimens on a timely and low-cost basis. Another prime- function of the Morphology Core will be to procure clinical-derived human samples with accurate demographic and diagnostic data. The continue availability of large, centralized bank of dermatopathologic and wound healing samples is a critical component and invaluable resource for investigators who conduct skin-related projects. The Director and all research assistants will supply teaching assistance and advice in microscopic techniques useful for skin analysis for P&F investigators, Funded SDRC investigators and their post-doctoral fellows, graduate students, and laboratory personnel. Cooperative interactions among the Molecular Genetics and Phenotype Cores will occur on a daily basis snap frozen tissues are delivered to the Molecular Genetics Core or animal samples and/or cultured cells are generated from the Phenotype Core.