EXCITOTOXIC & NEURONAL DYSFUNCTION IN BRAIN INJURY

这项研究的长期目标是建立一个更好的桥梁 组织、细胞和分子水平的分析之间的关系， 青蛙胚胎 本提案的具体目标是探讨 早期胚胎中分子表达域的性质。 的 pMRI跟踪三种组织运动和细胞谱系的能力 将使用不透明样本中随时间变化的尺寸， 用传统的分子生物学技术， 基因表达区域的基因， 这些细胞曾经在这个区域内。 具体假设 要测试的是，基因表达结构域是由 动态细胞群，需要细胞表达标记物 当他们进入一个给定的域，并消灭它，因为他们退出了 域 为此，我们将：（1）确定正常谱系 形成青蛙神经系统的细胞。 轴向 中胚层与近轴中胚层的关系：（2）确定 早期谱系和基因差异模式之间的联系 表达：（3）确定细胞谱系和细胞 扰动胚胎的运动 1. 目标导向成像：（a）提供全面的 在三个发育中大脑的三维活体图像， 不同种属（鹌鹑、小鼠和猴）的磁共振成像 (MR)显微成像，（B）使用功能磁共振成像技术来检查视觉 猫头鹰猴的野外地图随着 年轻动物脑中的髓鞘形成。 2. 分析：（a）在不同层次上分析图像 从简单的注释到将发病和发展 在空间和时间上组织不同的结构， 比较和对比鸟类，啮齿动物和哺乳动物系统。 (b)网格 同一动物的光学和MRI数据。 3. 传播：（a）制作图像和渲染工具， 以方便的格式向科学界提供分析。 例如，在一个实施例中，3D数据的2D切片的照片。 附有注释的光盘 使用现成的软件查看2D和3D图像， 内部开发的软件。 以及一个可通过以下途径访问的联机数据库： 在万维网上，原始数据和经过处理的数据将 可能是可用的。

The long-term goal of this research is to build a better bridge between the tissue, cellular, and molecular levels of analysis of the frog embryo. The specific goal of this proposal is to probe the nature of molecular expression domains in the early embryo. The ability of pMRI to follow tissue movements and cell lineages in three dimensions over time in opaque specimens will be used, in conjunction with conventional molecular biology techniques, to map the lineages that contribute to a gene expression domain as well as the fates of the cells that were once within the domain. The specific hypothesis to be tested is that the gene expression domains are made up of dynamic populations of cells, requiring that cells express the marker gene as they enter a given domain, and extinguish it as they exit the domain. Towards this end, we will: (1) determine the normal lineages of cells that contribute to the frog nervous system. the axial mesoderm and the paraxial mesoderm: (2) determine the rel ationship between the early lineages and differential patterns of gene expression: (3) determine the changes in cell lineages and cell movements in perturbed embryos 1. Goal Directed Imaging: (a) provide comprehensive three-dimensional in vivo images of the developing brain in three different species (quail, mouse, and monkey) using Magnetic Resonance (MR) micro-imaging, (b) use fMRI techniques to examine how the visual field map of the owl monkey changes with increasing degree of myelination in brain of the young animal. 2. Analysis: (a) analyze the images at a number of different levels ranging from simple annotation to correlating the onset and developing organization of different structures in both space & time and to compare & contrast the avian, rodent, and mammalian systems. (b) mesh optical and MRI data of the same animal. 3. Dissemination: (a) make the images and tools for rendering & analysis available to the scientific community in a convenient format. e.g., photographs of 2D slices of the 3D data. CD-ROMs with annotated 2D and 3D images to be viewed using readily available software and software developed in-house. and an on-line database accessible via the World Wide Web where raw' as well as 'processed' data will potentially be available.