CORE--ANALYTICAL CHEMISTRY CORE

分析化学核心的目的是支持计划项目 侦查人员配合尖端技术的开发和应用 化学分离、分级、结构测定、 和定量分析。核心的功能是提供成本- 个别实验室获得专业知识的有效手段 以及以其他方式在个别实验室无法获得的能力。这个 CORE将资助使用质谱学、色谱学和 整个计划项目的光谱技术。此外， CORE将支持改进分析技术的发展 包括免疫亲和分离、多通道近红外荧光 探测器、激光解吸质谱仪和原子力显微镜(AFM)。 针对基于近红外荧光的ELISA的开发进行的具体努力 用于包括硫代尿酸在内的暴露生物标志物的测定 和其他硫醚代谢物。紫外可见光谱和质量 光谱技术将被用来优化半抗原在 抗原偶联物和酶示踪剂。GC、HPLC、GC/MS和LC/MS 将使用技术来确认免疫分析结果并验证 化验性能。合成中间体的特征将使用 高分辨MS、MS/MS以及核磁共振、UV-Vis和FTIR光谱。 将为热学和生物学研究提供支持。 通过实施本地化和定量形态分析进行修复 有机、有机金属和无机副产品。激光解吸质量 光谱分析将用于金属的快速形态分析(例如 颗粒和固体样品中的有机污染物 包括土壤。原子吸收光谱分析、溶出伏安法、 X射线荧光、电子显微镜和原子力显微镜将补充这些 努力。微柱高效液相色谱/电喷雾质谱仪将用于表征 人绒毛膜促性腺激素的变异作为候选生物标记物 生殖功能以及萘的代谢物， 硝化萘、氯化乙烷和乙烯。电喷雾MS 并用激光解吸质谱仪对加合物进行了表征 具有特定蛋白质靶点的亲电代谢物。类似的方法 将被用来探索关键代谢的结构和表达 酯酶、水解酶和谷胱甘肽-S转移酶等蛋白质 靶组织和用于生物检测的生物体中的同工酶和 生物修复。包括柱在内的生物测定定向分级方案 将开发层析和超滤技术来探索 有机和无机物在蒸气之间的分配， 颗粒、胶体和溶解的相，因为这种分配是 预计将对运输、生物利用度和 毒性。分析核心还将提供指导和培训 为研究人员提供超级资金以开发采样方案， 实验设计、仪器操作和数据解释，以及 将采取行动促进超级基金项目之间的合作。

The aim of the Analytical Chemistry Core is to support Program Project investigators with the development and application of sophisticated methods of chemical isolation, fractionation, structure determination, and quantitative analysis. The function of the Core is to provide cost- efficient means for individual laboratories to obtain access to expertise and capabilities not otherwise available in individual laboratories. The Core will subsidize the use of mass spectrometric, chromatographic, and spectroscopic techniques for the entire program project. Furthermore, the Core will support the development of improved analytical technologies including immunoaffinity isolation, multichannel near-IR fluorescence detectors, laser desorption MS, and atomic force microscopy (AFM). Specific efforts target development of near-IR fluorescence-based ELISAs for determination of biomarkers of exposure including mercapturic acid and other thioether metabolites. UV-visible spectroscopic and mass spectrometric techniques will be used to optimize hapten loadings on antigenic conjugates and enzyme tracers. GC, HPLC, GC/MS, and LC/MS techniques will be employed to confirm immunoassay results and validate assay performance. Synthetic intermediates will be characterized using high resolution MS, MS/MS, and NMR, Uv-vis, and FTIR spectroscopy. Support will be provided for studies of thermal and biological remediation by performing localization and quantitative speciation of organic, organometallic, and inorganic byproducts. Laser desorption mass spectrometry will be employed for rapid speciation of metals (e.g. chromium) and organic contaminants in particulate and solid samples including soils. Atomic absorption spectroscopy, stripping voltammetry, x-ray fluorescence, electron microscopy, and AFM will complement these efforts. Microcolumn HPLC/electrospray MS methods will be to characterize variants of human chorionic gonadotropin as candidate biomarkers of reproductive function, as well as metabolites of naphthalene, nitronaphthalene, and chlorinated ethanes and ethenes. Electrospray MS and laser desorption MS will be employed to characterize adducts of electrophilic metabolites with specific protein targets. Similar methods will be used to probe the structures and expression of key metabolic proteins such as esterases, hydrolases, and glutathione-S-transferase isozymes in target tissues and also in organisms used for bioassays and bioremediation. Bioassay-directed fractionation schemes including column chromatography and ultrafiltration will be developed to explore partitioning of organic and inorganic substances between vapor, particulate, colloidal, and dissolved phases as this partitioning is expected to have profound effects upon transport, bioavailability, and toxicity. The Analytical Core will also provide instruction and training to Superfund researchers in development of sampling protocols, experimental design, instrument operation, and data interpretation, and will act to facilitate collaborations between Superfund projects.