CORE--ANALYTICAL CHEMISTRY CORE

分析化学核心的目的是支持计划项目 研究人员开发和应用先进的 化学分离、分馏、结构测定的方法， 和定量分析。核心的功能是提供成本- 各实验室获得专门知识的有效途径 以及个别实验室无法获得的能力。的 核心将补贴使用质谱，色谱， 整个项目的光谱技术。而且 核心将支持改进分析技术的开发 包括免疫亲和分离、多通道近红外荧光 检测器、激光解吸MS和原子力显微镜（AFM）。 具体努力的目标是开发基于近红外荧光的ELISA 用于确定接触的生物标志物，包括巯基尿酸 和其它硫醚代谢物。紫外可见光谱和质量 光谱技术将用于优化半抗原加载， 抗原缀合物和酶示踪剂。GC、HPLC、GC/MS和LC/MS 技术将用于确认免疫测定结果并验证 测定性能。合成中间体将采用以下方法进行表征： 高分辨率MS、MS/MS和NMR、UV-vis和FTIR光谱。 将为热能和生物学研究提供支助。 通过进行定位和定量形态的修复 有机、有机金属和无机副产物。激光解吸质谱 光谱法将用于金属的快速形态（例如， 铬）和有机污染物的颗粒和固体样品 包括土壤。原子吸收光谱法，溶出伏安法， X射线荧光、电子显微镜和原子力显微镜将补充这些技术 努力微柱HPLC/电喷雾MS方法将用于表征 人绒毛膜促性腺激素变异体作为 生殖功能，以及萘的代谢物， 硝基萘和氯化乙烷和乙烯。电喷雾MS 和激光解吸MS将用于表征的加合物 亲电代谢产物与特定蛋白质靶点。类似的方法 将用于探测关键代谢产物的结构和表达， 蛋白质如酯酶、水解酶和谷胱甘肽-S-转移酶 在靶组织中以及在用于生物测定的生物体中的同工酶， 生物修复生物测定导向的分馏方案，包括色谱柱 将开发色谱和超滤技术， 有机和无机物质在蒸汽之间的分配， 颗粒、胶体和溶解相，因为这种分配是 预计对运输、生物利用度和 毒性分析核心还将提供指导和培训 向超级基金研究人员提供采样协议的开发， 实验设计、仪器操作和数据解释，以及 将采取行动促进超级基金项目之间的合作。

The aim of the Analytical Chemistry Core is to support Program Project investigators with the development and application of sophisticated methods of chemical isolation, fractionation, structure determination, and quantitative analysis. The function of the Core is to provide cost- efficient means for individual laboratories to obtain access to expertise and capabilities not otherwise available in individual laboratories. The Core will subsidize the use of mass spectrometric, chromatographic, and spectroscopic techniques for the entire program project. Furthermore, the Core will support the development of improved analytical technologies including immunoaffinity isolation, multichannel near-IR fluorescence detectors, laser desorption MS, and atomic force microscopy (AFM). Specific efforts target development of near-IR fluorescence-based ELISAs for determination of biomarkers of exposure including mercapturic acid and other thioether metabolites. UV-visible spectroscopic and mass spectrometric techniques will be used to optimize hapten loadings on antigenic conjugates and enzyme tracers. GC, HPLC, GC/MS, and LC/MS techniques will be employed to confirm immunoassay results and validate assay performance. Synthetic intermediates will be characterized using high resolution MS, MS/MS, and NMR, Uv-vis, and FTIR spectroscopy. Support will be provided for studies of thermal and biological remediation by performing localization and quantitative speciation of organic, organometallic, and inorganic byproducts. Laser desorption mass spectrometry will be employed for rapid speciation of metals (e.g. chromium) and organic contaminants in particulate and solid samples including soils. Atomic absorption spectroscopy, stripping voltammetry, x-ray fluorescence, electron microscopy, and AFM will complement these efforts. Microcolumn HPLC/electrospray MS methods will be to characterize variants of human chorionic gonadotropin as candidate biomarkers of reproductive function, as well as metabolites of naphthalene, nitronaphthalene, and chlorinated ethanes and ethenes. Electrospray MS and laser desorption MS will be employed to characterize adducts of electrophilic metabolites with specific protein targets. Similar methods will be used to probe the structures and expression of key metabolic proteins such as esterases, hydrolases, and glutathione-S-transferase isozymes in target tissues and also in organisms used for bioassays and bioremediation. Bioassay-directed fractionation schemes including column chromatography and ultrafiltration will be developed to explore partitioning of organic and inorganic substances between vapor, particulate, colloidal, and dissolved phases as this partitioning is expected to have profound effects upon transport, bioavailability, and toxicity. The Analytical Core will also provide instruction and training to Superfund researchers in development of sampling protocols, experimental design, instrument operation, and data interpretation, and will act to facilitate collaborations between Superfund projects.