ELECTRON TRANSFER RATE BASED DETECTION OF LIGANDS

长程电子转移速率对供体-受体的几何形状以及中间介质的构象和运动非常敏感。因此，通过蛋白质的电子转移速率可能潜在地用作配体诱导的蛋白质结构和动力学变化的非常灵敏的探针。最近开发的组合技术提供了一种设计结合任何给定的靶分子的蛋白质的能力，这表明电子检测配体诱导的构象变化的方法可能提供一种监测任何特定底物存在的一般手段。我们在这里提出的实验，旨在监测配体诱导的折叠Hin重组酶通过蛋白质电子转移速率的变化。这些将证明一个通用的，电子转移为基础的电子技术用于检测特定的配体的药用意义的可行性。

Long-range electron transfer rates are exquisitely sensitive to donor-acceptor geometry and the conformation and motions of the intervening medium. The rate of electron transfer through proteins may thus potentially serve as a very sensitive probe of ligand- induced changes in protein structure and dynamics. Recently developed combinatorial techniques provide an ability to design proteins that bind to any given target molecule, suggesting that a method of electronically detecting ligand-induced conformational changes might provide a general means of monitoring the presence of any specific substrate. We propose here experiments aimed at monitoring the ligand-induced folding of Hin-recombinase via changes in through-protein electron transfer rate. These will demonstrate the feasibility of a general, electron-transfer based electronic technique for the detection of specific ligands of medicinal significance.