REAL TIME NONLINEAR RAMAN MICROSCOPE

有人建议开发一种新的仪器，用于生物医学研究和临床研究的广泛领域。该仪器是为实时、非侵入性显微成像而设计的。该装置由激光系统、显微镜和检测系统组成。提出了一种现代光谱技术--相干反斯托克斯拉曼散射--作为一种无创探头。介绍了一种新型激光器和光学设计，并对其进行了评价。为了提高拉曼成像的空间分辨率，提出了一种新的拉曼成像微观结构。提出了几种新的检测方案，融合了最先进的科学知识和技术成果。已经设计并将建造一台仪器的原型。将确定拟议仪器的技术规格。开发的仪器将在威斯康星大学密尔沃基分校用于细胞过程的生物物理研究。

It is proposed to develop a novel instrument for a broad area of biomedical research and clinical studies. This instrument is deigned for a real time, non-invasive microscopic imaging. The proposed apparatus consists of a laser system, a microscope, and detection system. A modern spectroscopic technique - coherent anti-stokes raman scattering - is suggested as a non-invasive probe. A novel laser and optical design is described and evaluated. A new microscopic arrangement for raman imaging is proposed in order to improve spatial resolution. Several novel detection schemes, incorporating both state-of-the-art scientific knowledge and technical achievements are suggested. A prototype of instruments is designed and will be constructed. Technical specifications for the proposed instrument will be determined. The developed instrument will be used at the University of Wisconsin-Milwaukee for biophysical studies of cellular processes.