REAL-TIME MICROSCOPIC IMAGING OF MEMBRANE POTENTIAL

描述(由申请人提供)：这项提议利用激光技术的进步，使检测电子设备成为可能。 在未染色的活组织中以非侵入性的方式活动。第一个目标是 建造一台可以检测聚焦产生的SH信号的激光显微镜 波长的激光辐射将使组织 损坏。光学和数据采集将被设计为在 一种非侵入性的方式，并最大限度地方便用户。第二个目标是 评估和测试显微镜的灵敏度，包括其空间和 经典动作电位检测中的时间分辨率 神经生理学准备。第三个目标是使用SH显微镜进行 发育中突触功能发育的先导性实验 脊椎动物的神经肌肉连接。这些研究可以提供 关于突触形成机制的信息，目前还不是 使用其他技术也是可能的。此外，衡量活动的能力 在神经肌肉交界处以一种非侵入性的方式 临床应用数量。像重症肌无力， 肌萎缩侧索硬化症、肌萎缩侧索硬化症和格林-巴利综合征影响神经细胞活性 神经和/或肌肉细胞。光学显微镜可能导致新的诊断 方法和更好地了解疾病进展的机制。

DESCRIPTION (provided by applicant): This proposal takes advantage of advances in laser technology that make it possible to detect electrical activity in unstained living tissue in a noninvasive manner. The first aim is to build a laser microscope that can detect SH signals generated by focused laser radiation at wavelengths that will minimize the possibility of tissue damage. The optics and data acquisition will be engineered for optimal use in a noninvasive manner and to maximize user-friendliness. The second aim is to evaluate and test the sensitivity of the microscope including its spatial and temporal resolution in detecting action potentials in a classical neurophysiological preparation. The third aim is to use the SH microscope for pilot tests on the functional development of synapses at the developing neuromuscular junction of vertebrates. These studies could provide information about the mechanisms of synapse formation that are not currently possible with other techniques. In addition, the ability to measure activity at the neuromuscular junction in a noninvasive way would have value in a number of clinical applications. Diseases like Myasthenia Gravis, the Muscular Dystrophies, ALS, and Guillain-Barr syndrome affect the activity of nerve and/or muscle cells. Optical microscopy could lead to new diagnostic methods and a better understanding of the mechanisms of disease progression.