DIDEOXY DNA SEQUENCING WITH TOF-MS

描述（改编自研究者摘要）：继续关注 寻找更快更便宜的DNA测序方法。 之一 几个实验室正在研究的方法是形成双脱氧测序 用质谱仪测量它们。 了一些成功 通过MALDI-MS测量短梯子，但不是长梯子， 后者正在通过电泳进行测序。 不幸的是， 为此目的的电泳是一种缓慢且昂贵的技术。 在 至少部分地，DNA分子片段化、脱嘌呤化和 在MALDI-MS中形成加合离子限制了该方法的性能。 在 该建议，提出了一种新的技术，可能会克服 这些问题，从而推进质谱的有用性， 双脱氧DNA测序梯的测量。

DESCRIPTION (Adapted from the Investigator's Abstract): Interest continues in finding faster and less expensive ways for sequencing DNA. One of the approaches under study in several laboratories is to form dideoxy sequencing ladders and measure them by mass spectrometry. Some success has been achieved in measuring short ladders by MALDI-MS, but not long ladders, so the latter are being sequenced by electrophoresis. Unfortunately, electrophoresis for this purpose is a slow and expensive technique. At least in part, the tendency of the DNA molecule to fragment, depurinate and form adduct ions in MALDI-MS has limited the performance of this method. In this proposal, a novel technique is presented which potentially may overcome these problems, and thereby advance the usefulness of mass spectrometry for the measurement of dideoxy DNA sequencing ladders.