ANTIBODY RESPONSES TO OLIGOMERIC HIV ENVELOPE FORMS: VACCINE

在发展中最可怕的障碍之一， 有效的抗艾滋病毒疫苗是我们缺乏知识，如何最好地 将HIV抗原呈递给免疫系统以产生保护性免疫 应答 具体地，通过免疫产生强有力的 动物和人类对HIV的中和抗体反应 包膜是HIV感染过程中免疫反应的天然靶点， 已被证明是一项极其艰巨的任务。 可以 之前的免疫尝试引发中和抗体 获得艾滋病毒信封未能这样做，因为信封形式 作为免疫原，与表面的存在不同， 感染性HIV病毒体或HIV感染细胞。 额外 缺乏中和抗体产生的可能性是 给药的包膜蛋白不能有效地 到适当的免疫细胞。 为了解决这些问题，我们 建议用一种HIV包膜免疫猕猴， 在原代HIV分离株表面发现的模拟物， 各种方法，以发挥其优化交付的潜力 我们的免疫原与相关的免疫细胞。 我们会监察 在血液和阴道粘膜中产生中和抗体。 基金AmFAR（02572）出版物无

One of the most formidable obstacles in the development of an effective anti-HIV vaccine is our lack of knowledge on how best to present HIV antigens to the immune system to develop protective immune responses. Specifically, the generation by immunization of vigorous neutralizing antibody responses in animals and humans against the HIV envelope, a natural target for immune responses during HIV infection, has been proven to be an extremely difficult tasks. It is possible that previous immunization attempts to elicit neutralizing antibodies gains the HIV envelope have failed to do so because the envelope form used as immunogen was different from the existing of the surface of infectious HIV virions or HIV-infected cells. AN additional possibility for the lack of generation of neutralizing antibodies is that the administered envelope proteins were inefficiently delivered to the appropriate immune cells. To address these possibilities, we propose to immunize macaques with a form of the HIV envelope that mimics that found on the surface of primary HIV isolates and test various methodologies for their potential to optimize the delivery of our immunogen to relevant immune cells. We will monitor the generation of neutralizing antibodies in the blood and vaginal mucosa. FUNDING AmFAR (02572) PUBLICATIONS None