ANTIBODY MEDIATED NEUTRALIZ HUMAN RHINOVIRUS 14 & BOVINE GLUTAMATE DEHYDROGENASE

我们已经确定了完整的人鼻病毒14的结构 与中和Fab片段复合，至少为4.5 决议。这个应用程序是为了更多的象棋时间来延长 通过改进冷冻条件或通过以下方式解决这些研究 通过将病毒与VH-VL片段复合。这表示 第一个完整的抗体中和病毒的晶体结构。 这一结构最终将解决由 Mandel在1976年的研究中指出，抗体只作用于病毒粒子，通过诱导 病毒粒子的构象变化。牛谷氨酸脱氢酶 是一种高度变构的同六聚体酶，催化 谷氨酸可逆氧化脱氨制α-酮戊二酸。这个 酶受到多种变构效应物的影响，具有不同寻常的 动力学性质。我们已经将这种酶结晶为流产的 与缓蚀剂GTP的络合物和晶体衍射到 ~2.7决议。这些脆弱的晶体需要同步加速器时间来 收集本地数据集。

We have determined the structure of intact human rhinovirus 14 complexed with neutralizing Fab fragments to ast least 4.5
resolution. This application is for more CHESS time to extend the resolution of these studies by either refining freezing conditions or by complexing the virus with VH-VL fragments. This represents the first crystal structure of an intact, antibody-neutralized virus. This structure will finally address the mechanism first proposed by Mandel in 1976 where antibodies act solely on virions by inducing conformational changes in the virion. Bovine glutamate dehydrogenase is a highly allosteric homo-hexameric enzyme that catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate. The enzyme is affected by numerous allosteric effectors and has unusual kinetic properties. We have crystallized the enzyme as an abortive complex along with the inhibitor GTP and the crystals diffract to ~2.7
resolution. These fragile crystals require synchrotron time to collect a native data set.