ANTIBODY MEDIATED NEUTRALIZ HUMAN RHINOVIRUS 14 & BOVINE GLUTAMATE DEHYDROGENASE

我们已经确定了完整的人鼻病毒14的结构 与中和Fab片段复合至至少4.5
分辨率 此应用程序是更多的国际象棋时间，以延长 通过改进冷冻条件或 通过将病毒与VH-VL片段复合。 这代表 完整的抗体中和病毒的第一个晶体结构。 这一结构将最终解决由 Mandel在1976年提出，抗体仅通过诱导 病毒体的构象变化。 牛谷氨酸脱氢酶 是一种高度变构的同源六聚体酶， 谷氨酸可逆氧化脱氨为α-酮戊二酸。 的 酶受许多变构效应物的影响， 动力学性质 我们已经把这种酶结晶为一种 复合物沿着抑制剂GTP和晶体的作用， ~2.7
分辨率 这些易碎的晶体需要同步加速器时间 收集本地数据集。

We have determined the structure of intact human rhinovirus 14 complexed with neutralizing Fab fragments to ast least 4.5
resolution. This application is for more CHESS time to extend the resolution of these studies by either refining freezing conditions or by complexing the virus with VH-VL fragments. This represents the first crystal structure of an intact, antibody-neutralized virus. This structure will finally address the mechanism first proposed by Mandel in 1976 where antibodies act solely on virions by inducing conformational changes in the virion. Bovine glutamate dehydrogenase is a highly allosteric homo-hexameric enzyme that catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate. The enzyme is affected by numerous allosteric effectors and has unusual kinetic properties. We have crystallized the enzyme as an abortive complex along with the inhibitor GTP and the crystals diffract to ~2.7
resolution. These fragile crystals require synchrotron time to collect a native data set.